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Title: Human neutrophils phagocytose and kill Acinetobacter baumannii and A. pittii
Authors: Lázaro-Díez, María
Chapartegui-González, Itziar
Redondo-Salvo, Santiago
Leigh, Chike
Merino Mata, David
San Segundo, David
Navas, Jesús
Icardo, José Manuel
Acosta Arbelo, Félix 
Ocampo-Sosa, Alain A.
Martínez-Martínez, Luis
Ramos-Vivas, José
UNESCO Clasification: 32 Ciencias médicas
Issue Date: 2017
Journal: Scientific Reports 
Abstract: Acinetobacter baumannii is a common cause of health care associated infections worldwide. A. pittii is an opportunistic pathogen also frequently isolated from Acinetobacter infections other than those from A. baumannii. Knowledge of Acinetobacter virulence factors and their role in pathogenesis is scarce. Also, there are no detailed published reports on the interactions between A. pittii and human phagocytic cells. Using confocal laser and scanning electron microscopy, immunofluorescence, and live-cell imaging, our study shows that immediately after bacteria-cell contact, neutrophils rapidly and continuously engulf and kill bacteria during at least 4 hours of infection in vitro. After 3 h of infection, neutrophils start to release neutrophil extracellular traps (NETs) against Acinetobacter. DNA in NETs colocalizes well with human histone H3 and with the specific neutrophil elastase. We have observed that human neutrophils use large filopodia as cellular tentacles to sense local environment but also to detect and retain bacteria during phagocytosis. Furthermore, co-cultivation of neutrophils with human differentiated macrophages before infections shows that human neutrophils, but not macrophages, are key immune cells to control Acinetobacter. Although macrophages were largely activated by both bacterial species, they lack the phagocytic activity demonstrated by neutrophils.
ISSN: 2045-2322
DOI: 10.1038/s41598-017-04870-8
Source: Scientific Reports [ISSN 2045-2322], v. 7 (1), article number 4571
Rights: by-nc-nd
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