|Title:||Sporulation and sterilization method for axenic culture of Gelidium canariensis||Authors:||Garcia-Jimenez, P.
Marián de Diego, Fernando
Rodrigo Sanz, Marta
Robaina, R. R.
|UNESCO Clasification:||241707 Algología (ficología)||Keywords:||Algas rojas
|Issue Date:||1999||Journal:||Progress in Industrial Microbiology||Abstract:||A sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2min). The branchlets were cultivated overnight in multiwell plates with 0.3 ml of autoclaved seawater to promote spore liberation in 90% of the cultivated branchlets. The branchlets were transferred to an antibiotic solution made of ampicillin, penicillin, rifampicin, nystatin (0.2 mg ml-1 each) and 0.1 g ml-1 of GeO2 in liquid PES for 45 days, during which clusters of spores (85 – 100 spores) were observed on the surface of the branchlet. After 55 days, they became axenic sporelings with the prostrate and erect system characteristic of Gelidium canariensis.||URI:||http://hdl.handle.net/10553/1729||ISSN:||0079-6352||DOI:||10.1016/S0079-6352(99)80117-9||Source:||Progress in Industrial Microbiology [ISSN 0079-6352],v. 35, p. 227-229|
|Appears in Collections:||Artículos|
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