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Title: Sporulation and sterilization method for axenic culture of Gelidium canariensis
Authors: Garcia-Jimenez, P. 
Marián de Diego, Fernando
Rodrigo Sanz, Marta
Robaina, R. R. 
UNESCO Clasification: 241707 Algología (ficología)
Keywords: Algas rojas
Algas marinas
Issue Date: 1999
Journal: Progress in Industrial Microbiology 
Abstract: A sporulation and sterilization procedure was used to establish axenic cultures of sporelings of Gelidium canariensis. Sporangial branchlets excised from the thallus were rinsed in distilled water twice and in 1% sodium hypochlorite (2min). The branchlets were cultivated overnight in multiwell plates with 0.3 ml of autoclaved seawater to promote spore liberation in 90% of the cultivated branchlets. The branchlets were transferred to an antibiotic solution made of ampicillin, penicillin, rifampicin, nystatin (0.2 mg ml-1 each) and 0.1 g ml-1 of GeO2 in liquid PES for 45 days, during which clusters of spores (85 – 100 spores) were observed on the surface of the branchlet. After 55 days, they became axenic sporelings with the prostrate and erect system characteristic of Gelidium canariensis.
ISSN: 0079-6352
DOI: 10.1016/S0079-6352(99)80117-9
Source: Progress in Industrial Microbiology [ISSN 0079-6352],v. 35, p. 227-229
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