Please use this identifier to cite or link to this item: https://accedacris.ulpgc.es/handle/10553/143347
Title: Pilot Transcriptomic Profiling of Canine Oral Melanoma Reveals Conserved Oncogenic Pathways and Uncharacterized Molecular Signatures
Authors: Perez-Santana, Carmen G.
Rodríguez-Esparragon, Francisco 
Cazorla-Rivero, Sara E.
Jimenez-Alonso, Ana A.
Clavo Varas, Bernardino 
Gonzalez-Martin, Jesus M.
Canovas-Molina, Angeles
Bartolome, Carmen
Estupinan, Lidia
Rodríguez Grau-Bassas, Enrique 
UNESCO Clasification: 310910 Cirugía
310907 Patología
Keywords: Malignant-Melanoma
Expression
Mutations
Filaggrin
Canine Oral Melanoma, et al.
Issue Date: 2025
Journal: Cancers (Basel) 
Abstract: Background: Canine oral melanoma (COM) is an aggressive and often fatal neoplasm in dogs, with clinical and molecular similarities to human melanoma. Despite its relevance as a comparative oncology model, the molecular mechanisms underlying COM remain poorly understood. This study aimed to characterize gene expression profiles in COM to identify differentially expressed genes (DEGs), potential biomarkers, and therapeutic targets. Methods: In this pilot study, we performed RNA sequencing (RNA-seq) on tumor and healthy oral tissue samples from dogs. Two independent analytical pipelines-Bowtie2-DESeq2 and HISAT-StringTie-Ballgown-were used to ensure robustness in DEG detection. We also conducted pathway enrichment and isoform-level analyses to investigate biological processes and alternative splicing events. Results: Both approaches identified a core set of 929 common DEGs. Key oncogenic pathways, including MAPK/ERK and cell cycle regulation, were significantly affected, with notable upregulation of BRAF, NRAS, CDK4, and MITF (log2FC = 2.86, p < 0.001). The transcription factor SOX10 and the cytokine IL-33, both previously implicated in melanoma progression, were consistently overexpressed. Additionally, NF1, a known RAS pathway inhibitor, was also upregulated. Isoform analysis revealed novel transcript variants, suggesting a complex layer of post-transcriptional regulation in COM. Many DEGs remained uncharacterized, and chromosomal distribution analysis highlighted potential genomic influences. Conclusions: Our findings provide new insights into the molecular landscape of COM, reinforcing its utility as a model for human melanoma. The identification of conserved oncogenic pathways and novel transcript variants opens avenues for further functional studies and the development of targeted therapies in both veterinary and human oncology.
URI: https://accedacris.ulpgc.es/handle/10553/143347
ISSN: 2072-6694
DOI: 10.3390/cancers17132106
Fuente: Cancers [2072-6694], v. 17 (13), (Junio 2025)
Colección:Artículos
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