Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/136608
Título: Dolphin Pituitary Gland: Immunohistochemistry and Ultrastructural Cell Characterization Following a Novel Anatomical Dissection Protocol and Non-Invasive Imaging (MRI)
Autores/as: Alonso Almorox, Paula 
Blanco, Alfonso
Fiorito, Carla
Sierra Pulpillo, Eva María 
Suarez Santana, Cristian Manuel 
Consoli, Francesco 
Arbelo Hernández, Manuel Antonio 
Guzmán, Raiden Grandía
Molpeceres Diego, Ignacio 
Fernández Gómez, Antonio 
Almunia Portolés, Javier 
Castro Alonso, Ayoze 
Fernández Rodríguez, Antonio Jesús 
Clasificación UNESCO: 310907 Patología
240119 Zoología marina
330723 Dispositivos de rayos x
Palabras clave: pituitary
adenohypophysis
neurohypophysis
odontocetes
comparative anatomy, et al.
Fecha de publicación: 2025
Publicación seriada: Animals 
Resumen: The pituitary gland regulates essential physiological processes in mammals. De- spite its importance, research on its anatomy and ultrastructure in dolphins remains scarce. Using non-invasive imaging technology (MRI) and a novel skull-opening and dissection protocol, this study characterizes the dolphin pituitary through immunohistochemistry (IHC) and transmission electron microscopy (TEM). A total of 47 pituitaries were collected from stranded common bottlenose dolphins (Tursiops truncatus). common dolphins (Del- phinus delphis), and Atlantic spotted dolphins (Stenella frontalis). as well as from captive common bottlenose dolphins. MRI allowed visualization of the gland’s anatomy and its spatial relationship with the hypothalamus and surrounding structures. A modified skull-opening and pituitary extraction protocol ensured the preservation of the adenohy- pophysis and neurohypophysis for detailed analysis. Histological, immunohistochemical, and ultrastructural studies confirmed the gland’s structural organization, identifying eight distinct adenohypophyseal cell types: corticotrophs (ACTH), somatotrophs (GH), go- nadotrophs (FSH and LH), lactotrophs (LTH), melanotrophs (MSH), thyrotrophs (TSH), follicular cells, and capsular cells. This study presents the first immunolabelling of thy- rotrophs in cetacean adenohypophysis and the first detailed ultrastructural characterization of adenohypophyseal cells in cetaceans, providing baseline data for future research. By integrating multidisciplinary techniques, it advances the understanding of dolphin neu- roendocrinology and highlights broader implications for cetacean health, welfare, and conservation
URI: http://hdl.handle.net/10553/136608
ISSN: 2076-2615
DOI: 10.3390/ani15050735
Fuente: Animals[ISSN2076-2615], v.15 (Marzo 2025)
Colección:Artículos
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