Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/132333
Title: Targeting plasmid-encoded proteins that contain immunoglobulin-like domains to combat antimicrobial resistance
Authors: Miró, Luïsa
Prieto, Alejandro
Margolles, Yago
Bernabeu, Manuel
Salguero, David
Merino, Susana
Tomas, Joan
Corbera Sánchez, Juan Alberto 
Perez-Bosque, Anna
Huttener, Mario
Fernández, Luis Ángel
Juarez, Antonio
UNESCO Clasification: 240111 Patología animal
310905 Microbiología
Keywords: Enterica
Infectious Disease
Microbiology
Mouse
Salmonella, et al
Issue Date: 2024
Journal: eLife 
Abstract: Antimicrobial resistance (AMR) poses a significant threat to human health. Although vaccines have been developed to combat AMR, it has proven challenging to associate specific vaccine antigens with AMR. Bacterial plasmids play a crucial role in the transmission of AMR. Our recent research has identified a group of bacterial plasmids (specifically, IncHI plasmids) that encode large molecular mass proteins containing bacterial immunoglobulin-like domains. These proteins are found on the external surface of the bacterial cells, such as in the flagella or conjugative pili. In this study, we show that these proteins are antigenic and can protect mice from infection caused by an AMR Salmonella strain harboring one of these plasmids. Furthermore, we successfully generated nanobodies targeting these proteins, that were shown to interfere with the conjugative transfer of IncHI plasmids. Considering that these proteins are also encoded in other groups of plasmids, such as IncA/C and IncP2, targeting them could be a valuable strategy in combating AMR infections caused by bacteria harboring different groups of AMR plasmids. Since the selected antigens are directly linked to AMR itself, the protective effect extends beyond specific microorganisms to include all those carrying the corresponding resistance plasmids.
URI: http://hdl.handle.net/10553/132333
ISSN: 2050-084X
DOI: 10.7554/eLife.95328.3
Source: eLife[EISSN 2050-084X],v. 13, (Julio 2024)
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