Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/128785
Title: Comparative study of <i>BCR-ABL1</i> quantification: Xpert assay, a feasible solution to standardization concerns
Authors: López-Jorge, CE
Gómez-Casares, MT
Jiménez-Velasco, A
García-Bello, MA
Barrios, M
Lopez, J
De La Iglesia Íñigo, Silvia Narcisa 
Ramírez, T
Sánchez, G
Heiniger, AI
Molero Labarta, María Teresa 
UNESCO Clasification: 32 Ciencias médicas
320708 Hematología
Keywords: BCR-ABL
QRT-PCR
CML
MDR
Issue Date: 2012
Journal: Annals of Hematology 
Abstract: The level of BCR-ABL1 reached after treatment with tyrosine kinase inhibitors is an effective marker of the therapeutic response and a good survival predictor in chronic myeloid leukemia (CML) patients. However, no agreement has yet been achieved about either the standardization of the technique to determine BCR-ABL1 or the interpretation of the results. The aim of this study was to compare the method currently recommended by the European LeukemiaNet,which includes the application of a conversion factor to express the results in international scale, with an automated method (Xpert BCR-ABL™, Cepheid). BCR-ABL1 transcript quantification was performed in 117 samples from CML patients in two different laboratories by both methods, and the results were compared by statistical procedures. A high linear correlation was obtained in the results between the two methods. The concordance at logarithmic intervals reached 62 %. When the major molecular response (MMR) was analyzed, 85 % agreement was achieved. The automated method provides reproducible results and does not show significant differences compared with the traditional method. As a clinical tool, Xpert correctly classified the patients inMMR and can be considered a useful alternative for the molecular follow-up of CML patients.
URI: http://hdl.handle.net/10553/128785
ISSN: 0939-5555
DOI: 10.1007/s00277-012-1468-4
Source: Annals of Hematology [0939-5555], v. 91, pág. 1245-1250 (abril 2012)
Appears in Collections:Artículos
Show full item record

SCOPUSTM   
Citations

18
checked on Mar 30, 2025

WEB OF SCIENCETM
Citations

17
checked on Mar 30, 2025

Page view(s)

75
checked on Nov 16, 2024

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.