Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/128785
Título: Comparative study of <i>BCR-ABL1</i> quantification: Xpert assay, a feasible solution to standardization concerns
Autores/as: López-Jorge, CE
Gómez-Casares, MT
Jiménez-Velasco, A
García-Bello, MA
Barrios, M
Lopez, J
De La Iglesia Íñigo, Silvia Narcisa 
Ramírez, T
Sánchez, G
Heiniger, AI
Molero Labarta, María Teresa 
Clasificación UNESCO: 32 Ciencias médicas
320708 Hematología
Palabras clave: BCR-ABL
QRT-PCR
CML
MDR
Fecha de publicación: 2012
Publicación seriada: Annals of Hematology 
Resumen: The level of BCR-ABL1 reached after treatment with tyrosine kinase inhibitors is an effective marker of the therapeutic response and a good survival predictor in chronic myeloid leukemia (CML) patients. However, no agreement has yet been achieved about either the standardization of the technique to determine BCR-ABL1 or the interpretation of the results. The aim of this study was to compare the method currently recommended by the European LeukemiaNet,which includes the application of a conversion factor to express the results in international scale, with an automated method (Xpert BCR-ABL™, Cepheid). BCR-ABL1 transcript quantification was performed in 117 samples from CML patients in two different laboratories by both methods, and the results were compared by statistical procedures. A high linear correlation was obtained in the results between the two methods. The concordance at logarithmic intervals reached 62 %. When the major molecular response (MMR) was analyzed, 85 % agreement was achieved. The automated method provides reproducible results and does not show significant differences compared with the traditional method. As a clinical tool, Xpert correctly classified the patients inMMR and can be considered a useful alternative for the molecular follow-up of CML patients.
URI: http://hdl.handle.net/10553/128785
ISSN: 0939-5555
DOI: 10.1007/s00277-012-1468-4
Fuente: Annals of Hematology [0939-5555], v. 91, pág. 1245-1250 (abril 2012)
Colección:Artículos
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