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Title: Improvements in the Modeling and Kinetics Processes of the Enzymatic Synthesis of Pentyl Acetate
Authors: Lorenzo Pérez, Beatriz 
Fernández Suárez, Luis Jesús 
Ortega Saavedra, Juan 
Domínguez Déniz, Leandro 
UNESCO Clasification: 221003 Cinética química
Keywords: Esterification
Pentyl acetate
Kinetics modeling
Lipozyme (R) 435
Solvent-free system
Issue Date: 2023
Journal: Processes 
Abstract: In this work, the enzymatic synthesis of pentyl acetate obtained from acetic acid and pentan-1-ol using the commercial immobilized lipase Lipozyme®435 was studied. Specifically, the effects of several variables of the process on the kinetics were shown, such as the initial concentration of the acetic acid, the alcohol/acid molar ratio, and the possible reuse of the enzyme, while other variables, such as temperature, agitation, and the enzyme/acid ratio were held constant. The kinetics were determined by assessing the acetic acid concentration throughout the reactive process. Experimental data were correlated with the rate equation consisting of a modified version of the Bi–Bi Ping-Pong mechanism. The results showed that when no hydrophobic solvents were used with the reagents in stoichiometric proportion, a high molar fraction of acetic acid (x0,acid ≈ 0.50) caused the loss of enzymatic activity, achieving a conversion of only 5%. However, when there was an excess of pentan-1-ol, the reaction occurred successfully. Under optimal conditions (solvent-free conditions, x0,alcohol/x0,acid = 2, and x0,acid = 0.33), it was found that the enzyme could be reused up to 10 times without a loss of activity, reaching conversions higher than 80% after 8 h. Therefore, those conditions are advantageous in terms of productivity.
ISSN: 2227-9717
DOI: 10.3390/pr11061640
Source: Process [ISSN 2227-9717], v. 11, nº 6, 1640, 2023
Appears in Collections:Artículos
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checked on Jun 16, 2024

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