Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/123707
Title: Validation of a Method Scope Extension for Simple Biomonitoring of 353 Pollutants in Serum Samples
Authors: Rial Berriel, Cristian Javier 
Ramos Luzardo, Álvaro 
Acosta Dacal, Andrea Carolina 
Macías Montes, Ana 
Fernández Valerón, Josefa Pilar 
Henríquez Hernández, Luis Alberto 
Zumbado Peña, Manuel Luis 
Domínguez Boada, Luis María 
Pérez Luzardo, Octavio Luis 
UNESCO Clasification: 32 Ciencias médicas
3214 Toxicología
3212 Salud pública
Keywords: Serum
POPs
Pesticides
Biomonitoring
Pharmaceuticals
Issue Date: 2023
Journal: Toxics 
Abstract: Animals and humans are exposed to various residues that can have a detrimental impact on health, including carcinogenic potential, endocrine disruption, or fatal toxicity. The toxic burden can be evaluated in several biological samples, with serum being one of the preferred and most convenient options. In this study, we have applied and validated a method for detecting several hundred toxins in serum samples. This technique involved a single-step QuEChERS (quick, easy, cheap, effective, rugged, and safe) extraction followed by analysis using gas and liquid chromatography coupled with mass spectrometry. With this methodology, we could detect and quantify up to 353 compounds, including persistent organic pollutants (POPs), pesticides, pharmaceuticals, and rodenticides, using just 250 µL of serum. Among them, 92% could be measured at concentrations below 1.25 ng/mL, making it ideal for biomonitoring. We applied this method to samples collected from camels (n = 40) and humans (n = 25). We detected naproxen, ketoprofen, paracetamol, levamisole, and some POPs in these samples. This study validated the ability to simultaneously detect a broad range of compounds in small volumes of serum.
URI: http://hdl.handle.net/10553/123707
ISSN: 2305-6304
DOI: 10.3390/toxics11060498
Source: Toxics [ISSN 2305-6304], v. 11 (6), 498, (Mayo 2023)
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