Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/123431
Título: The effects of diet and addition of Conjugated Linoleic Acid (CLA) on goat kid complement system activity.
Autores/as: Morales De La Nuez, Antonio José 
Moreno Indias,Isabel 
Castro Navarro, Noemí 
Sánchez Macías,Davinia 
Capote Álvarez, Juan Francisco 
Ruiz Díaz, M.D.
Argüello Henríquez, Anastasio 
Clasificación UNESCO: 3104 Producción Animal
Fecha de publicación: 2010
Editor/a: International Goat Association
Conferencia: 10th International Conference on Goats - Technological Development and Associate Attempts to a Sustainable Small Livestock Activity 
Resumen: 40 newborn goat kids of Majorera breed (20 males and 20 females) were used for evaluating the effect of Conjugated Linoleic Acid (CLA) milk replacer addition in Complement System Activity (CSA). The animals were separated of their dam’s immediately after birth and were randomly distributed in four groups. Goat kids were fed with frozen-thawed goat colostrum by bottle-feeding at 2, 14 and 26 hours of life. After colostrum feeding period, the control group (MR) received a commercial milk replacer, the MG group was fed with milk goat, the CLA-2 group received milk replacer plus 2 ml of CLA-60 and the CLA-4 group received milk replacer plus 4 ml of CLA-60. All animals were fed twice daily until day 60 after birth. To determine the CSA effect, blood samples were collected every 24 hours from day 1 to day 5 and at day 10, 20, 30, 40, 50 and 60 of life. Blood samples were centrifuged and the blood plasma fraction was frozen at -80ºC until analysis. The CSA was measured using DGHB++ buffer (Dextrose, Gelatin, Hepes) to evaluate total CSA and Mg-EGTA-DGHB buffer to determine CSA alternative pathway. Alternative and total CSA did not differ in our study and increased throughout the experimental period in all groups. MR goat kids showed the lowest threshold CSA until 40 days of life (by 0-15%), while threshold values in MG group was observed until day 10; for the CLA-2 this threshold was found until day 5 of the experiment; nevertheless in the CLA-4 group the activity increased at day 3 of life. In all experimental groups the highest values were observed at 50 and 60 days of life. CSA did not differed significantly at 1, 2, 3, 4, and 10 days of life in all groups; however at day 5, CSA was significantly higher in CLA-4 than in control goat kids. MG had significantly higher CSA at day 50 than MR, CLA-2 and CLA-4. The CSA reached similar values at 2 moths of life for MG, CLA-2 and CLA-4 groups; however MR group presented lower CSA at this time. In conclusion, the CLA addition to goat milk replacer improves the complement system activity in goat kids in a similar way that animals fed with goat milk after 2 months of development.
URI: http://hdl.handle.net/10553/123431
Fuente: 10th International Conference on Goats
Colección:Ponencias
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