Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/121602
Título: Laser capture microdissection as a method for investigating the human hair follicle microbiome reveals region-specific differences in the bacteriome profile
Autores/as: Lousada, Marta B.
Edelkamp, J.
Lachnit, T.
Fehrholz, M.
Jiménez Acosta, F. 
Paus, R.
Clasificación UNESCO: 32 Ciencias médicas
320106 Dermatología
Palabras clave: 16S rRNA Sequencing
Hair Follicles
Microbiome
Fecha de publicación: 2023
Publicación seriada: BMC Research Notes 
Resumen: Objective: Human hair follicles (HFs) are populated by a rich and diverse microbiome, traditionally evaluated by methods that inadvertently sample the skin microbiome and/or miss microbiota located in deeper HF regions. Thereby, these methods capture the human HF microbiome in a skewed and incomplete manner. This pilot study aimed to use laser-capture microdissection of human scalp HFs, coupled with 16S rRNA gene sequencing to sample the HF microbiome and overcome these methodological limitations. Results: HFs were laser-capture microdissected (LCM) into three anatomically distinct regions. All main known core HF bacterial colonisers, including Cutibacterium, Corynebacterium and Staphylococcus, were identified, in all three HF regions. Interestingly, region-specific variations in α-diversity and microbial abundance of the core microbiome genera and Reyranella were identified, suggestive of variations in microbiologically relevant microenvironment characteristics. This pilot study therefore shows that LCM-coupled with metagenomics is a powerful tool for analysing the microbiome of defined biological niches. Refining and complementing this method with broader metagenomic techniques will facilitate the mapping of dysbiotic events associated with HF diseases and targeted therapeutic interventions.
URI: http://hdl.handle.net/10553/121602
ISSN: 1756-0500
DOI: 10.1186/s13104-023-06302-5
Fuente: BMC Research Notes [EISSN 1756-0500], v. 16 (1), 29, (Diciembre 2023)
Colección:Comentario
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