Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/121602
Campo DC Valoridioma
dc.contributor.authorLousada, Marta B.en_US
dc.contributor.authorEdelkamp, J.en_US
dc.contributor.authorLachnit, T.en_US
dc.contributor.authorFehrholz, M.en_US
dc.contributor.authorJiménez Acosta, F.en_US
dc.contributor.authorPaus, R.en_US
dc.date.accessioned2023-03-28T07:47:16Z-
dc.date.available2023-03-28T07:47:16Z-
dc.date.issued2023en_US
dc.identifier.issn1756-0500en_US
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/121602-
dc.description.abstractObjective: Human hair follicles (HFs) are populated by a rich and diverse microbiome, traditionally evaluated by methods that inadvertently sample the skin microbiome and/or miss microbiota located in deeper HF regions. Thereby, these methods capture the human HF microbiome in a skewed and incomplete manner. This pilot study aimed to use laser-capture microdissection of human scalp HFs, coupled with 16S rRNA gene sequencing to sample the HF microbiome and overcome these methodological limitations. Results: HFs were laser-capture microdissected (LCM) into three anatomically distinct regions. All main known core HF bacterial colonisers, including Cutibacterium, Corynebacterium and Staphylococcus, were identified, in all three HF regions. Interestingly, region-specific variations in α-diversity and microbial abundance of the core microbiome genera and Reyranella were identified, suggestive of variations in microbiologically relevant microenvironment characteristics. This pilot study therefore shows that LCM-coupled with metagenomics is a powerful tool for analysing the microbiome of defined biological niches. Refining and complementing this method with broader metagenomic techniques will facilitate the mapping of dysbiotic events associated with HF diseases and targeted therapeutic interventions.en_US
dc.languageengen_US
dc.relation.ispartofBMC Research Notesen_US
dc.sourceBMC Research Notes [EISSN 1756-0500], v. 16 (1), 29, (Diciembre 2023)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320106 Dermatologíaen_US
dc.subject.other16S rRNA Sequencingen_US
dc.subject.otherHair Folliclesen_US
dc.subject.otherMicrobiomeen_US
dc.titleLaser capture microdissection as a method for investigating the human hair follicle microbiome reveals region-specific differences in the bacteriome profileen_US
dc.typeinfo:eu-repo/semantics/annotationen_US
dc.typeAnnotationen_US
dc.identifier.doi10.1186/s13104-023-06302-5en_US
dc.identifier.scopus85149936057-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.orcidNO DATA-
dc.contributor.authorscopusid57222001594-
dc.contributor.authorscopusid57216536629-
dc.contributor.authorscopusid26536238100-
dc.contributor.authorscopusid35745837300-
dc.contributor.authorscopusid58140448500-
dc.contributor.authorscopusid34572423200-
dc.identifier.eissn1756-0500-
dc.identifier.issue1-
dc.relation.volume16en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Comentarioen_US
dc.utils.revisionen_US
dc.date.coverdateDiciembre 2023en_US
dc.identifier.ulpgcen_US
dc.identifier.ulpgcen_US
dc.identifier.ulpgcen_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr0,534
dc.description.sjrqQ2
dc.description.miaricds9,6
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR IUIBS: Patología médica-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameJimenez Acosta,Francisco-
Colección:Comentario
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