Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/121020
Título: Efficient Oral Priming of Tenebrio molitor Larvae Using Heat-Inactivated Microorganisms
Autores/as: Gonzalez-Acosta, S
Baca-Gonzalez, V
Asensio-Calavia, P
Otazo-Perez, A
Lopez, MR
Morales De La Nuez, Antonio José 
de la Lastra, JMP
Clasificación UNESCO: 310905 Microbiología
Palabras clave: Immune priming
Antimicrobial peptides
Invertebrate immunity
Mealworms
In vitro antimicrobial assay, et al.
Fecha de publicación: 2022
Publicación seriada: Vaccines 
Resumen: Microbial resistance is a global health problem that will increase over time. Advances in insect antimicrobial peptides (AMPs) offer a powerful new approach to combat antimicrobial resistance. Invertebrates represent a rich group of animals for the discovery of new antimicrobial agents due to their high diversity and the presence of adaptive immunity or “immune priming”. Here, we report a priming approach for Tenebrio molitor that simulates natural infection via the oral route. This oral administration has the advantage of minimizing the stress caused by conventional priming techniques and could be a viable method for mealworm immunity studies. When using inactivated microorganisms for oral priming, our results showed an increased survival of T. molitor larvae after exposure to various pathogens. This finding was consistent with the induction of antimicrobial activity in the hemolymph of primed larvae. Interestingly, the hemolymph of larvae orally primed with Escherichia coli showed constitutive activity against Staphylococcus aureus and heterologous activity for other Gram-negative bacteria, such as Salmonella enterica. The priming of T. molitor is generally performed via injection of the microorganism. To our knowledge, this is the first report describing the oral administration of heat-inactivated microorganisms for priming mealworms. This technique has the advantage of reducing the stress that occurs with the conventional methods for priming vertebrates.
URI: http://hdl.handle.net/10553/121020
ISSN: 2076-393X
DOI: 10.3390/vaccines10081296
Fuente: Vaccines [ISSN 2076-393X], v. 10 (8)
Colección:Artículos
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