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Title: Vaccination Upregulates Th1 Cytokines in the Lung of Pigs Experimentally Infected with Mycoplasma hyopneumoniae
Authors: Rodríguez Guisado, Francisco 
Rosales Santana, Rubén Sebastián 
Ramírez Corbera, Ana Sofía 
Poveda Guerrero, José Bismarck 
UNESCO Clasification: 310907 Patología
310903 Inmunología
240110 Histología animal
Keywords: Cytokines
Mycoplasma Hyopneumoniae
Porcine, et al
Issue Date: 2023
Journal: Animals 
Abstract: Mycoplasma hyopneumoniae (Mhy) is the causative agent of enzootic pneumonia, characterized by high morbidity and low mortality rates in intensive swine production systems. To better understand the mechanisms underlying the protection of an inactivated whole cell vaccine, we investigated the immunohistochemical differences in the cytokine expression in vaccinated and non-vaccinated pigs experimentally infected with Mhy. Four-week-old Mhy-negative pigs (n = 24) were allocated to negative control (n = 8), or one of two Mhy-infected groups: vaccinated (n = 8) and non-vaccinated (n = 8). Infection was carried out by a combination of trans-tracheal and aerosol route. Lung samples were processed for histopathological and immunohistochemical studies, by using antibodies against Mhy, IL1-α, IL1-β, IL-2, IL-4, IL-5, IL-6, Il-8, IL-10, IL-12p35, IL-13, IL-17A, TNF-α, IFN-γ, and CD-4 lymphocytes. Although all cytokines increased in both infected groups, IL-2, IL-12, and IFN-γ were significantly overexpressed in vaccinated pigs. These findings, in conjunction with the decrease of macroscopic and histological lesions in vaccinated animals, indicate the importance to enhance Th1 response in the immunization strategies to control Mhy infection.
ISSN: 2076-2615
DOI: 10.3390/ani13030520
Source: Animals [EISSN 2076-2615], v. 13 (3), (Febrero 2023)
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