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Title: Genetics and Nutrition Drive the Gut Microbiota Succession and Host-Transcriptome Interactions through the Gilthead Sea Bream (Sparus aurata) Production Cycle
Authors: Naya-Català, Fernando
Piazzon, M. Carla
Torrecillas Burriel,Silvia 
Toxqui-Rodríguez, Socorro
Calduch-Giner, Josep A.
Fontanillas, Ramón
Sitjà-Bobadilla, Ariadna
Montero Vítores, Daniel 
Pérez-Sánchez, Jaume
UNESCO Clasification: 310502 Piscicultura
240109 Crecimiento animal
240108 Genética animal
Keywords: Epithelial Turnover
Gut Microbiota
Host Transcriptomics
Inflammatory Response
Intestinal Motility, et al
Issue Date: 2022
Project: Genomic and nutritional innovations for genetically superior farmed fish to improve efficiency in European aquaculture 
Journal: Biology 
Abstract: Fish genetically selected for growth (GS) and reference (REF) fish were fed with CTRL (15% FM, 5–7% FO) or FUTURE (7.5% FM, 10% poultry meal, 2.2% poultry oil + 2.5% DHA-algae oil) diets during a 12-months production cycle. Samples from initial (t0; November 2019), intermediate (t1; July 2020) and final (t2; November 2020) sampling points were used for Illumina 16S rRNA gene amplicon sequencing of the adherent microbiota of anterior intestine (AI). Samples from the same individuals (t1) were also used for the gene expression profiling of AI by RNA-seq, and subsequent correlation analyses with microbiota abundances. Discriminant analyses indicated the gut bacterial succession along the production cycle with the proliferation of some valuable taxa for facing seasonality and different developmental stages. An effect of genetic background was evidenced along time, decreasing through the progression of the trial, namely the gut microbiota of GS fish was less influenced by changes in diet composition. At the same time, these fish showed wider transcriptomic landmarks in the AI to cope with these changes. Our results highlighted an enhanced intestinal sphingolipid and phospholipid metabolism, epithelial turnover and intestinal motility in GS fish, which would favour their improved performance despite the lack of association with changes in gut microbiota composition. Furthermore, in GS fish, correlation analyses supported the involvement of different taxa with the down-regulated expression of pro-inflammatory markers and the boosting of markers of extracellular remodelling and response to bacterium. Altogether, these findings support the combined action of the gut microbiome and host transcriptionally mediated effects to preserve and improve gut health and function in a scenario of different growth performance and potentiality.
ISSN: 2079-7737
DOI: 10.3390/biology11121744
Source: Biology [EISSN 2079-7737], v. 11 (12), 1744, (Diciembre 2022)
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