Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/119393
Campo DC Valoridioma
dc.contributor.authorMartín Rodríguez, Alberto Jonatanen_US
dc.contributor.authorGonzález-Orive, A.en_US
dc.contributor.authorHernández-Creus, A.en_US
dc.contributor.authorFlores Morales, Amilcaren_US
dc.contributor.authorDorta-Guerra, R.en_US
dc.contributor.authorNorte, M.en_US
dc.contributor.authorMartín, V.S.en_US
dc.contributor.authorFernández, J.J.en_US
dc.date.accessioned2022-11-25T15:23:29Z-
dc.date.available2022-11-25T15:23:29Z-
dc.date.issued2014en_US
dc.identifier.isbn14712180-
dc.identifier.issn1471-2180en_US
dc.identifier.urihttp://hdl.handle.net/10553/119393-
dc.description.abstractBackground: A variety of conditions (culture media, inocula, incubation temperatures) are employed in antifouling tests with marine bacteria. Shewanella algae was selected as model organism to evaluate the effect of these parameters on: bacterial growth, biofilm formation, the activity of model antifoulants, and the development and nanomechanical properties of the biofilms.The main objectives were:.1) To highlight and quantify the effect of these conditions on relevant parameters for antifouling studies: biofilm morphology, thickness, roughness, surface coverage, elasticity and adhesion forces.2) To establish and characterise in detail a biofilm model with a relevant marine strain. Results: Both the medium and the temperature significantly influenced the total cell densities and biofilm biomasses in 24-hour cultures. Likewise, the IC 50 of three antifouling standards (TBTO, tralopyril and zinc pyrithione) was significantly affected by the medium and the initial cell density. Four media (Marine Broth, MB; 2% NaCl Mueller-Hinton Broth, MH2; Luria Marine Broth, LMB; and Supplemented Artificial Seawater, SASW) were selected to explore their effect on the morphological and nanomechanical properties of 24-h biofilms. Two biofilm growth patterns were observed: a clear trend to vertical development, with varying thickness and surface coverage in MB, LMB and SASW, and a horizontal, relatively thin film in MH2. The Atomic Force Microscopy analysis showed the lowest Young modulii for MB (0.16 ± 0.10 MPa), followed by SASW (0.19 ± 0.09 MPa), LMB (0.22 ± 0.13 MPa) and MH2 (0.34 ± 0.16 MPa). Adhesion forces followed an inverted trend, being higher in MB (1.33 ± 0.38 nN) and lower in MH2 (0.73 ± 0.29 nN). Conclusions: All the parameters significantly affected the ability of S. algae to grow and form biofilms, as well as the activity of antifouling molecules. A detailed study has been carried out in order to establish a biofilm model for further assays. The morphology and nanomechanics of S. algae biofilms were markedly influenced by the nutritional environments in which they were developed. As strategies for biofilm formation inhibition and biofilm detachment are of particular interest in antifouling research, the present findings also highlight the need for a careful selection of the assay conditions.en_US
dc.languageengen_US
dc.relation.ispartofBMC Microbiologyen_US
dc.sourceBMC Microbiology [1471-2180], v. 14:102 (abril 2014)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320103 Microbiología clínicaen_US
dc.subject.otherAdhesion forcesen_US
dc.subject.otherAntifoulingen_US
dc.subject.otherAtomic Force Microscopyen_US
dc.subject.otherBiofilmen_US
dc.subject.otherBiofoulingen_US
dc.subject.otherCLSM analysisen_US
dc.subject.otherYoung modulusen_US
dc.titleOn the influence of the culture conditions in bacterial antifouling bioassays and biofilm properties: Shewanella algae, a case studyen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1186/1471-2180-14-102en_US
dc.identifier.scopus2-s2.0-84899990743-
dc.contributor.orcid#NODATA#-
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dc.identifier.issue1-
dc.relation.volume14en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.external80016506-
dc.description.numberofpages15en_US
dc.utils.revisionen_US
dc.date.coverdateAbril 2014en_US
dc.identifier.ulpgcNoen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr1,472
dc.description.jcr2,729
dc.description.sjrqQ1
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextopen-
item.fulltextCon texto completo-
crisitem.author.deptGIR Investigación Básica y Aplicada en Ciencias de la Salud-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.orcid0000-0003-2422-129X-
crisitem.author.orcid0000-0002-0828-8921-
crisitem.author.parentorgDepartamento de Ciencias Clínicas-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameMartín Rodríguez, Alberto Jonatan-
crisitem.author.fullNameFlores Morales,Amilcar-
Colección:Artículos
Artículos
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