Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/119356
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dc.contributor.authorMartín Rodríguez, Alberto Jonatanen_US
dc.contributor.authorVillion, Katiaen_US
dc.contributor.authorYilmaz-Turan, Secilen_US
dc.contributor.authorVilaplana, Franciscoen_US
dc.contributor.authorSjöling, Åsaen_US
dc.contributor.authorRömling, Uteen_US
dc.date.accessioned2022-11-24T13:35:57Z-
dc.date.available2022-11-24T13:35:57Z-
dc.date.issued2021en_US
dc.identifier.issn1751-7907en_US
dc.identifier.urihttp://hdl.handle.net/10553/119356-
dc.description.abstractBacterial colony morphology can reflect different physiological stages such as virulence or biofilm formation. In this work we used transposon mutagenesis to identify genes that alter colony morphology and cause differential Congo Red (CR) and Brilliant Blue G (BBG) binding in Shewanella algae, a marine indigenous bacterium and occasional human pathogen. Microscopic analysis of colonies formed by the wild-type strain S. algae CECT 5071 and three transposon integration mutants representing the diversity of colony morphotypes showed production of biofilm extracellular polymeric substances (EPS) and distinctive morphological alterations. Electrophoretic and chemical analyses of extracted EPS showed differential patterns between strains, although the targets of CR and BBG binding remain to be identified. Galactose and galactosamine were the preponderant sugars in the colony biofilm EPS of S. algae. Surface-associated biofilm formation of transposon integration mutants was not directly correlated with a distinct colony morphotype. The hybrid sensor histidine kinase BarA abrogated surface-associated biofilm formation. Ectopic expression of the kinase and mutants in the phosphorelay cascade partially recovered biofilm formation. Altogether, this work provides the basic analysis to subsequently address the complex and intertwined networks regulating colony morphology and biofilm formation in this poorly understood species.en_US
dc.languageengen_US
dc.relation.ispartofMicrobial Biotechnologyen_US
dc.sourceMicrobial Biotechnology [ISSN 1751-7907] v. 14 (3), p. 1183-1200, (Marzo 2021)en_US
dc.subject32 Ciencias médicasen_US
dc.subject320103 Microbiología clínicaen_US
dc.subject.otherShewanella algaeen_US
dc.subject.otherBiofilmen_US
dc.subject.otherExtracellular polymeric substancesen_US
dc.subject.otherColony morphotypesen_US
dc.titleRegulation of colony morphology and biofilm formation in Shewanella algaeen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1111/1751-7915.13788en_US
dc.identifier.pmid33764668-
dc.identifier.scopus2-s2.0-85103052177-
dc.contributor.orcid#NODATA#-
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dc.description.lastpage1200en_US
dc.identifier.issue3-
dc.identifier.pmcidPMC8085958-
dc.description.firstpage1183en_US
dc.relation.volume14en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.identifier.external95308996-
dc.description.numberofpages18en_US
dc.utils.revisionen_US
dc.date.coverdateMarzo 2021en_US
dc.identifier.ulpgcNoen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.sjr1,106
dc.description.jcr6,575
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
dc.description.miaricds9,6
item.grantfulltextopen-
item.fulltextCon texto completo-
crisitem.author.deptGIR Investigación Básica y Aplicada en Ciencias de la Salud-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.orcid0000-0003-2422-129X-
crisitem.author.parentorgDepartamento de Ciencias Clínicas-
crisitem.author.fullNameMartín Rodríguez, Alberto Jonatan-
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