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Title: A recently isolated human commensal Escherichia coli ST10 clone member mediates enhanced thermotolerance and tetrathionate respiration on a P1 phage-derived IncY plasmid
Authors: Kamal, Shady Mansour
Cimdins-Ahne, Annika
Lee, Changhan
Li, Fengyang
Martín Rodríguez, Alberto Jonatan 
Seferbekova, Zaira
Afasizhev, Robert
Wami, Haleluya Tesfaye
Katikaridis, Panagiotis
Meins, Lena
Lunsdorf, Heinrich
Dobrindt, Ulrich
Mogk, Axel
Romling, Ute
UNESCO Clasification: 32 Ciencias médicas
320103 Microbiología clínica
320102 Genética clínica
Keywords: Disaggregase ClpG
Escherichia coli
IncY plasmid
Phylogenetic analysis
Tetrathionate respiration, et al
Issue Date: 2021
Journal: Molecular Microbiology 
Abstract: The ubiquitous human commensal Escherichia coli has been well investigated through its model representative E. coli K-12. In this work, we initially characterized E. coli Fec10, a recently isolated human commensal strain of phylogroup A/sequence type ST10. Compared to E. coli K-12, the 4.88 Mbp Fec10 genome is characterized by distinct single-nucleotide polymorphisms and acquisition of genomic islands. In addition, E. coli Fec10 possesses a 155.86 kbp IncY plasmid, a composite element based on phage P1. pFec10 harbours multiple cargo genes such as coding for a tetrathionate reductase and its corresponding regulatory two-component system. Among the cargo genes is also the Transmissible Locus of Protein Quality Control (TLPQC), which mediates tolerance to lethal temperatures in bacteria. The disaggregase ClpGGI of TLPQC constitutes a major determinant of the thermotolerance of E. coli Fec10. We confirmed stand-alone disaggregation activity, but observed distinct biochemical characteristics of ClpGGI-Fec10 compared to the nearly identical Pseudomonas aeruginosa ClpGGI-SG17M. Furthermore, we noted a unique contribution of ClpGGI-Fec10 to the exquisite thermotolerance of E. coli Fec10, suggesting functional differences between both disaggregases in vivo. Detection of thermotolerance in 10% of human commensal E. coli isolates hints to the successful establishment of food-borne heat-resistant strains in the human gut.
ISSN: 0950-382X
DOI: 10.1111/mmi.14614
Source: Molecular Microbiology [ISSN 0950-382X], v. 115 (2), p. 255-271, (Febrero 2021)
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