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Title: Isolation and Characterization of vB_kpnM_17-11, a Novel Phage Efficient Against Carbapenem-Resistant Klebsiella pneumoniae
Authors: Bai, Jiawei
Zhang, Feiyang
Liang, Shuang
Chen, Qiao
Wang, Wei
Wang, Ying
Martín Rodríguez, Alberto Jonatan 
Sjöling, Åsa
Hu, Renjing
Zhou, Yingshun
UNESCO Clasification: 32 Ciencias médicas
320103 Microbiología clínica
Keywords: Animal model
Klebsiella pneumonia
Phage therapy
Issue Date: 2022
Journal: Frontiers in cellular and infection microbiology 
Abstract: Phages and phage-encoded proteins exhibit promising prospects in the treatment of Carbapenem-Resistant Klebsiella pneumoniae (CRKP) infections. In this study, a novel Klebsiella pneumoniae phage vB_kpnM_17-11 was isolated and identified by using a CRKP host. vB_kpnM_17-11 has an icosahedral head and a retractable tail. The latent and exponential phases were 30 and 60 minutes, respectively; the burst size was 31.7 PFU/cell and the optimal MOI was 0.001. vB_kpnM_17-11 remained stable in a wide range of pH (4-8) and temperature (4-40°C). The genome of vB_kpnM_17-11 is 165,894 bp, double-stranded DNA (dsDNA), containing 275 Open Reading Frames (ORFs). It belongs to the family of Myoviridae, order Caudovirales, and has a close evolutionary relationship with Klebsiella phage PKO111. Sequence analysis showed that the 4530 bp orf022 of vB_kpnM_17-11 encodes a putative depolymerase. In vitro testing demonstrated that vB_kpnM_17-11 can decrease the number of K. pneumoniae by 105-fold. In a mouse model of infection, phage administration improved survival and reduced the number of K. pneumoniae in the abdominal cavity by 104-fold. In conclusion, vB_kpnM_17-11 showed excellent in vitro and in vivo performance against K. pneumoniae infection and constitutes a promising candidate for the development of phage therapy against CRKP.
ISSN: 2235-2988
DOI: 10.3389/fcimb.2022.897531
Source: Frontiers in cellular and infection microbiology [ISSN 2235-2988], v. 12, 897531, (Julio 2022)
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