Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/119277
Título: In vivo Bioluminescence Imaging of Luciferase-labeled Cancer Cells
Autores/as: Carceles-Cordon, Marc
Rodríguez Fernández, Irene 
Rodriguez-Bravo, Veronica
Cordon-Cardo, Carlos
Domingo-Domenech, Josep
Clasificación UNESCO: 32 Ciencias médicas
320713 Oncología
3302 Tecnología bioquímica
3314 Tecnología médica
Palabras clave: Bioluminiscence imaging
In Vivo
Mice
Cancer cells
Tumour
Fecha de publicación: 2016
Publicación seriada: Bio-Protocol 
Resumen: Over the past decade, in vivo bioluminescent imaging has emerged as a non-invasive and sensitive tool for studying ongoing biological processes within living organisms (Contag et al., 1997; Contag et al., 1998). Based on the detection and quantitation of the photons produced by the oxidation of luciferin by luciferase enzymes (Harvey, 1927), this technique has proved to be particularly useful in analyzing cancerous cells and monitoring tumor growth (Edinger et al., 1999; Sweeney et al., 1999; Vidal et al., 2015), providing a cost-effective insight into how the disease progresses in vivo, without the need of serial sacrifice of animals. This protocol describes in detail the procedure of obtaining luciferase-tagged tumors in immunocompromised mice that can be studied by bioluminescent imaging through the use of an IVIS Spectrum imager.
URI: http://hdl.handle.net/10553/119277
ISSN: 2331-8325
DOI: 10.21769/BioProtoc.1762
Fuente: Bio-Protocol [ISSN 2331-8325], v. 6 (6), e1762, (2016)
Colección:Artículos
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