Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/118739
Title: Diacylglycerol rather than Ca<sup>2+</sup> mediates GnRH inhibition of FSH induced steroidogenesis in ovarian granulosa cells
Authors: Fanjul Rodríguez, Luisa Fernanda 
Déniz Cáceres, Antonio 
Quintana Aguiar, José Martín 
Santana Delgado, María Del Pino 
Gonzalez, Juan
Estévez Rosas, Francisco Jesús 
Ruiz De Galarreta Hernandez,C. Manuel 
UNESCO Clasification: 32 Ciencias médicas
2302 Bioquímica
Keywords: Diacyglycerol
Steroidogenesis
Ovarian granulosa cells
Issue Date: 1992
Journal: Biochemical and biophysical research communications (Print) 
Abstract: Treatment of cultured granulosa cells with PLC or GnRH stimulated the rapid generation of DAG and phosphoinositide turnover. The PKC activators PLC (3 mU/ml) and TPA (10-7M) or the decapeptide GnRH (10-6M) elicited similar inhibitory responses on FSH or cAMP stimulated granulosa cell steroidogenesis. Mobilization of intracellular Ca2+ with A23187 (10-8M) was followed by a slight increase in the steroidogenic activity of cultured granulosa cells, whereas elevation of extracellular K+ (50 mM) largely augmented the steroid biosynthetic activity of the granulosa cells. These results suggest that the inhibitory effect of GnRH on granulosa cell steroidogenesis is mediated by generation of DAG, rather than by increases in intracellular Ca2+ concentrations.
URI: http://hdl.handle.net/10553/50584
http://hdl.handle.net/10553/118739
ISSN: 0006-291X
DOI: 10.1016/0006-291X(92)92369-9
Source: Biochemical and Biophysical Research Communications[ISSN 0006-291X],v. 188(1), p. 198-204 (Octubre 1992)
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