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http://hdl.handle.net/10553/114617
Title: | Nanobodies Protecting from Lethal SARS-CoV-2 Infection Target Receptor Binding Epitopes Preserved in Virus Variants other than Omicron | Authors: | Casasnovas, José M. Margolles, Yago Noriega, María A. Guzmán, María Arranz, Rocío Melero, Roberto Casanova, Mercedes Corbera Sánchez, Juan Alberto Jiménez-de-Oya, Nereida Gastaminza, Pablo Garaigorta, Urtzi Saiz, Juan Carlos Martín-Acebes, Miguel Ángel Fernández, Luis Ángel |
UNESCO Clasification: | 310903 Inmunología | Keywords: | Coronavirus Covid-19 Nanobodies Neutralizing Antibodies Sars-Cov-2 Variants |
Issue Date: | 2022 | Project: | PIE-RD-COVID 19 (No 202020E079) PTI+ Salud Global REC_EU (No SGL 2103051, NextGenerationEU) No SGL 2103053, NextGenerationEU CRIOMECORR project (ESFRI-2019-01-CSIC-16) Instruct Image Processing Center PID16168 and PID14989 |
Journal: | Frontiers in Immunology | Abstract: | The emergence of SARS-CoV-2 variants that escape from immune neutralization are challenging vaccines and antibodies developed to stop the COVID-19 pandemic. Thus, it is important to establish therapeutics directed toward multiple or specific SARS-CoV-2 variants. The envelope spike (S) glycoprotein of SARS-CoV-2 is the key target of neutralizing antibodies (Abs). We selected a panel of nine nanobodies (Nbs) from dromedary camels immunized with the receptor-binding domain (RBD) of the S, and engineered Nb fusions as humanized heavy chain Abs (hcAbs). Nbs and derived hcAbs bound with subnanomolar or picomolar affinities to the S and its RBD, and S-binding cross-competition clustered them in two different groups. Most of the hcAbs hindered RBD binding to its human ACE2 (hACE2) receptor, blocked cell entry of viruses pseudotyped with the S protein and neutralized SARS-CoV-2 infection in cell cultures. Four potent neutralizing hcAbs prevented the progression to lethal SARS-CoV-2 infection in hACE2-transgenic mice, demonstrating their therapeutic potential. Cryo-electron microscopy identified Nb binding epitopes in and out the receptor binding motif (RBM), and showed different ways to prevent virus binding to its cell entry receptor. The Nb binding modes were consistent with its recognition of SARS-CoV-2 RBD variants; mono and bispecific hcAbs efficiently bound all variants of concern except omicron, which emphasized the immune escape capacity of this latest variant. | URI: | http://hdl.handle.net/10553/114617 | ISSN: | 1664-3224 | DOI: | 10.3389/fimmu.2022.863831 | Source: | Frontiers in Immunology [EISSN 1664-3224], v. 13, (Abril 2022) |
Appears in Collections: | Artículos |
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