Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/114302
Título: Extracellular Polymeric Substances Produced by the Thermophilic Cyanobacterium Gloeocapsa gelatinosa: Characterization and Assessment of Their Antioxidant and Metal-Chelating Activities
Autores/as: Gongi, Wejdene
Gómez Pinchetti, Juan Luis 
Cordeiro, Nereida
Ouada, Hatem Ben
Clasificación UNESCO: 3302 Tecnología bioquímica
241707 Algología (ficología)
221090 Química-física de polímeros
Palabras clave: Gloeocapsa gelatinosa
Extracellular polymeric substances
Physicochemical characterization
Functional properties
Antioxidant activity, et al.
Fecha de publicación: 2022
Proyectos: Red de Excelencia en Biotecnología Azul (Algas) de la Región Macaronésica. Consolidación, Certificación y Transferencia 
Implementation and Sustainability of Microbial Resource Research Infrastructure for 21st Century 
Publicación seriada: Marine Drugs 
Resumen: Cyanobacteria, particularly thermophilic strains, represent an important potential source of EPSs, harboring structural complexity that predicts diverse and specific bioactive potential. The thermophilic cyanobacteria Gloeocapsa gelatinosa, isolated from a natural hot source in Ain Echfa (Tunisia), was cultivated in a cylindrical reactor, and the production of biomass and EPSs was investigated. Results revealed that the strain is amongst the most efficient EPSs producers (0.89 g L−1) and that EPSs production was not correlated with the growth phase. EPSs were sulfated heteropolysaccharides containing carbohydrates (70%) based on nine different monosaccharides, mainly mannose (22%), and with the presence of two uronic acids. EPSs were formed by two polymers moieties with a molecular weight of 598.3 ± 7.2 and 67.2 ± 4.4 kDa. They are thermostable in temperatures exceeding 100 °C and have an anionic nature (zeta potential of −40 ± 2 mV). Atomic force microscopy showed that EPSs formed multimodal lumps with 88 nm maximum height. EPSs presented high water holding capacity (70.29 ± 2.36%) and solubility index (97.43 ± 1.24%), and a strong bivalent metal sorption capacity especially for Cu2+ (91.20 ± 1.25%) and Fe2+ (75.51 ± 0.71%). The antioxidant activity of G. gelatinosa EPSs was investigated using four methods: the β-carotene-bleaching activity, DPPH assays, iron-reducing activity, and metal-chelating activity. EPS has shown high potential as free radicals’ scavenger, with an IC50 on DPPH (0.2 g L−1) three-fold lower than ascorbic acid (0.6 g L −1) and as a metal chelating activity (IC50 = 0.4 g L−1) significantly lower than EDTA. The obtained results allow further exploration of the thermophilic G. gelatinosa for several biotechnological and industrial applications.
URI: http://hdl.handle.net/10553/114302
ISSN: 1660-3397
DOI: 10.3390/md20040227
Fuente: Marine Drugs [ISSN 1660-3397], v. 20(4), 227, (Marzo 2022)
Colección:Artículos
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