Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/114205
Title: In vivo evaluation of additively manufactured multi-layered scaffold for the repair of large osteochondral defects
Authors: Tamaddon, Maryam
Blunn, Gordon
Tan, Rongwei
Yang, Pan
Sun, Xiaodan
Chen, Shen Mao
Luo, Jiajun
Liu, Ziyu
Wang, Ling
Li, Dichen
Donate González, Ricardo 
Monzón Verona, Mario Domingo 
Liu, Chaozong
UNESCO Clasification: 3314 Tecnología médica
Keywords: Additive Manufacturing
Large Animal
Osteochondral Scaffold
Porous Titanium
Issue Date: 2022
Project: Versus Arthritis (No. 21160)
Rosetree Trust (No. A1184)
Biomaterials and Additive Manufacturing: Osteochondral Scaffold innovation applied to osteoarthritis 
Innovate UK via Newton Fund (No. 102872)
Engineering and Physical Science Research Council (No. EP/T517793/1)
Journal: Bio-design and manufacturing 
Abstract: The repair of osteochondral defects is one of the major clinical challenges in orthopaedics. Well-established osteochondral tissue engineering methods have shown promising results for the early treatment of small defects. However, less success has been achieved for the regeneration of large defects, which is mainly due to the mechanical environment of the joint and the heterogeneous nature of the tissue. In this study, we developed a multi-layered osteochondral scaffold to match the heterogeneous nature of osteochondral tissue by harnessing additive manufacturing technologies and combining the established art laser sintering and material extrusion techniques. The developed scaffold is based on a titanium and polylactic acid matrix-reinforced collagen “sandwich” composite system. The microstructure and mechanical properties of the scaffold were examined, and its safety and efficacy in the repair of large osteochondral defects were tested in an ovine condyle model. The 12-week in vivo evaluation period revealed extensive and significantly higher bone in-growth in the multi-layered scaffold compared with the collagen–HAp scaffold, and the achieved stable mechanical fixation provided strong support to the healing of the overlying cartilage, as demonstrated by hyaline-like cartilage formation. The histological examination showed that the regenerated cartilage in the multi-layer scaffold group was superior to that formed in the control group. Chondrogenic genes such as aggrecan and collagen-II were upregulated in the scaffold and were higher than those in the control group. The findings showed the safety and efficacy of the cell-free “translation-ready” osteochondral scaffold, which has the potential to be used in a one-step surgical procedure for the treatment of large osteochondral defects.
URI: http://hdl.handle.net/10553/114205
ISSN: 2096-5524
DOI: 10.1007/s42242-021-00177-w
Source: Bio-Design and Manufacturing[ISSN 2096-5524], (Enero 2022)
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