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Title: Oligosaccharides production by enzymatic hydrolysis of banana pseudostem pulp
Authors: Díaz, Sara 
Ortega, Zaida 
Benítez, Antonio N. 
Marrero, María D. 
Carvalheiro, Florbela
Duarte, Luís C.
Matsakas, Leonidas
Krikigianni, Eleni
Rova, Ulrika
Christakopoulos, Paul
Fernandes, Maria C.
UNESCO Clasification: 3303 ingeniería y tecnología químicas
Keywords: Banana Wastes
Multi-Stage Enzymatic Hydrolysis
Issue Date: 2021
Project: Solutions through the new use for a waste of banana crop to develop products in aquaculture and plastics sector 
MED is a research unit funded by National Funds through Foundation for Science and Technology (FCT) from Portugal under the Project (UIDB/05183/2020)
Journal: Biomass Conversion and Biorefinery 
Abstract: Banana production generates significant amounts of agricultural wastes, being fiber extraction one of the most relevant alternatives for their valorization. This process produces banana’s pseudostem pulp (BPP) as a byproduct, which shows an interesting composition for the biorefinery’s biochemical platform, with high polysaccharides (68%) and low lignin contents. This work deals with the enzymatic hydrolysis (EH) of raw and hydrothermally pre-treated BPP, focusing on the production of oligosaccharides (OS). Raw BPP hydrolysis with cellulase at different dosages rendered only 3.2% OS yields (OSY). Pectinase addition has not affected EH performance. On the other hand, EH of hydrothermally pre-treated BPP at 150 °C and 170 °C (P150 and P170) allowed to increase OSY up to 28% (P150, 1 FPU of cellulase/g dry biomass, 12 h), being 72% of the solubilized sugars in the form of cello-oligosaccharides. This last condition was subjected to a multi-stage EH strategy without improvements in OSY. An endo-glucanase was also tested, but obtained OSY were lower than cellulase results. Finally, obtained OS demonstrated to stimulate the growth of two Lactobacilli strains. The results show that BPP pre-treated under mild operational conditions is a good candidate for cello-oligosaccharides production by EH using 1 FPU/g DB of cellulase with a simple strategy.
ISSN: 2190-6815
DOI: 10.1007/s13399-021-02033-4
Source: Biomass Conversion and Biorefinery [ISSN 2190-6815], (Enero 2021)
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