|Title:||Magnetite Nanoparticles Biomineralization in the Presence of the Magnetosome Membrane Protein MamC: Effect of Protein Aggregation and Protein Structure on Magnetite Formation||Authors:||Lopez-Moreno, Rafael
Valverde Tercedor, María Del Carmen
Azuaga Fortes, Ana I.
Casares Atienza, Salvador
Rodriguez-Navarro, Alejandro B.
|UNESCO Clasification:||32 Ciencias médicas
Nanoparticles, et al
|Issue Date:||2017||Journal:||Crystal Growth & Design||Abstract:||MamC from Magnetococcus marinus MC-1 has been shown to control the size of magnetite crystals in in vitro experiments, thereby demonstrating its potential as a candidate protein for the production of magnetite nanoparticles possibly useful in medical and other applications. However, the importance of the structure and aggregation state of the protein on the resulting biomimetic nanoparticles has not yet been assessed. One method normally used to prevent the aggregation of integral membrane proteins is the introduction of detergents during protein purification. In this study, results from protein aggregation following the addition of Triton-X100, DDM, and LDAO are presented. Magnetite particles formed in the presence of MamC purified using these three detergents were compared. Our results show that detergents alter the structure of the folded recombinant protein, thus preventing the ability of MamC to control the size of magnetite crystals formed chemically in vitro. Furthermore, we show that the introduction of detergents only at the dialysis process during the protein purification prevents its aggregation and allows for correct, functional folding of MamC. These results also indicate that the population of the active protein particles present at a certain oligomeric state needs to be considered, rather than only the oligomeric state, in order to interpret the ability of magnetosome recombinant proteins to control the size and/or morphology of magnetite crystals formed chemically in vitro.||URI:||http://hdl.handle.net/10553/111921||ISSN:||1528-7483||DOI:||10.1021/acs.cgd.6b01643||Source:||Crystal Growth & Design [ISSN 1528-7483], v. 17(4), p. 1620-1629|
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