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Title: Magnetite Nanoparticles Biomineralization in the Presence of the Magnetosome Membrane Protein MamC: Effect of Protein Aggregation and Protein Structure on Magnetite Formation
Authors: Lopez-Moreno, Rafael
Fernández-Vivas, Antonia
Valverde Tercedor, María Del Carmen 
Azuaga Fortes, Ana I.
Casares Atienza, Salvador
Rodriguez-Navarro, Alejandro B.
Zarivach, Raz
Jimenez-Lopez, Concepcion
UNESCO Clasification: 32 Ciencias médicas
2302 Bioquímica
2414 Microbiología
Keywords: Crystals
Nanoparticles, et al
Issue Date: 2017
Journal: Crystal Growth & Design 
Abstract: MamC from Magnetococcus marinus MC-1 has been shown to control the size of magnetite crystals in in vitro experiments, thereby demonstrating its potential as a candidate protein for the production of magnetite nanoparticles possibly useful in medical and other applications. However, the importance of the structure and aggregation state of the protein on the resulting biomimetic nanoparticles has not yet been assessed. One method normally used to prevent the aggregation of integral membrane proteins is the introduction of detergents during protein purification. In this study, results from protein aggregation following the addition of Triton-X100, DDM, and LDAO are presented. Magnetite particles formed in the presence of MamC purified using these three detergents were compared. Our results show that detergents alter the structure of the folded recombinant protein, thus preventing the ability of MamC to control the size of magnetite crystals formed chemically in vitro. Furthermore, we show that the introduction of detergents only at the dialysis process during the protein purification prevents its aggregation and allows for correct, functional folding of MamC. These results also indicate that the population of the active protein particles present at a certain oligomeric state needs to be considered, rather than only the oligomeric state, in order to interpret the ability of magnetosome recombinant proteins to control the size and/or morphology of magnetite crystals formed chemically in vitro.
ISSN: 1528-7483
DOI: 10.1021/acs.cgd.6b01643
Source: Crystal Growth & Design [ISSN 1528-7483], v. 17(4), p. 1620-1629
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