Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/111185
Title: Screening of new potential probiotics strains against Photobacterium damselae subsp. Piscicida for marine aquaculture
Authors: Gutiérrez Falcón, Ana Isabel 
Padilla Castillo, Daniel Fermín 
Real Valcárcel, Fernando 
Ramos Sosa, María José 
Acosta Hernández, Begoña María 
Sánchez Henao, Julián Andrés 
García Álvarez, Natalia 
Rosario Medina, Mª Inmaculada 
Silva Sergent, Freddy Alejandro 
Déniz Suárez, María Soraya 
Martín Barrasa, José Luis 
UNESCO Clasification: 310905 Microbiología
310801 Bacterias
310502 Piscicultura
Keywords: Marine aquaculture
Pasteurellosis
Probiotics
Issue Date: 2021
Project: Nuevos Priobioticos y Sus Productos Para El Control de Enfermedades en Especies Relevantes Pra la Acuicultura Marina y Continental 
Journal: Animals 
Abstract: On intensive fish farms, 10% of the population dies exclusively from pathogens, and Photo-bacterium damselae subsp. Piscicida (Ph. damselae subsp. Piscicida), the bacteria causing pasteurellosis in marine aquaculture, is one of the major pathogens involved. The objective of this study was to obtain new probiotic strains against pasteurellosis in order to limit the use of chemotherapy, avoiding the environmental repercussions generated by the abusive use of these products. In this study, 122 strains were isolated from the gills and intestines of different marine fish species and were later evaluated in vitro to demonstrate the production of antagonistic effects, the production of antibacterial sub-stances, adhesion and growth to mucus, resistance to bile and resistance to pH gradients, as well as its harmlessness and the dynamic of expression of immune-related genes by real-time PCR after administration of the potential probiotic in the fish diet. Only 1/122 strains showed excellent results to be considered as a potential probiotic strain and continue its characterization against Ph. damselae subsp. piscicida to determine its protective effect and elucidating in future studies its use as a possible probiotic strain for marine aquaculture.
URI: http://hdl.handle.net/10553/111185
ISSN: 2076-2615
DOI: 10.3390/ani11072029
Source: Animals [ISSN 2076-2615], v. 11(7), (Julio 2021)
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