Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/110940
Title: Retrograde flushing collection and freezing of dromedary camel epididymal spermatozoa with seminal plasma
Authors: Monaco, Davide
Batista Arteaga, Miguel 
Amann, Olga
Padalino, Barbara
Pieters, Wouter
Morelli, Mariacristina
Accogli, Gianluca
Desantis, Salvatore
Lacalandra, Giovanni Michele
UNESCO Clasification: 310411 Reproducción
Keywords: Dromedary camel
Epididymides
Kinematic parameters
Post-thaw
Seminal plasma
Issue Date: 2020
Journal: Acta Veterinaria Hungarica 
Abstract: The objectives of this study were to describe the parameters of dromedary camel epididymal spermatozoa collected by retrograde flushing (RF) technique and to evaluate the freezability of the collected sperm, diluted with and without the supplementation of seminal plasma (SP). Two experiments were conducted: in Experiment 1, ES were recovered within 6-8 h after castration; selected samples were diluted with a Tris-citrate egg-yolk glycerolated buffer and frozen. In Experiment 2, epididymides were stored for 24 h at 4 °C before RF and semen samples were frozen after dilution with a Tris-lactose eggyolk glycerolated extender with and without 15% SP. In Experiment 1, eight semen samples were obtained from ten epididymides with a mean of 500 × 106 total spermatozoa recovered, per flushed epididymis. Mean post-thaw motility and progressive motility were 75 and 17%, respectively. In Experiment 2, 15 samples were collected, out of the 18 epididymides (mean number of collected spermatozoa: 700 ×106), and 13 of these samples were of excellent quality. Post-thaw parameters were not satisfactory but the supplementation of the freezing medium with 15% SP improved the progressive motility and kinematic parameters of the spermatozoa.
URI: http://hdl.handle.net/10553/110940
ISSN: 0236-6290
DOI: 10.1556/004.2020.00042
Source: Acta Veterinaria Hungarica [ISSN 0236-6290], v. 68(3), p. 289-297, (Septiembre 2020)
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