Please use this identifier to cite or link to this item:
Title: Dietary Phytogenics and Galactomannan Oligosaccharides in Low Fish Meal and Fish Oil-Based Diets for European Sea Bass (Dicentrarchus labrax) Juveniles: Effects on Gill Structure and Health and Implications on Oxidative Stress Status
Authors: Torrecillas Burriel,Silvia 
Terova, Genciana
Makol, Alex
Serradell Pastor, Antonio 
Valdenegro Vega, Victoria
Izquierdo López, María Soledad 
Acosta Arbelo, Félix Antonio 
Montero Vítores, Daniel 
UNESCO Clasification: 310502 Piscicultura
310406 Nutrición
251092 Acuicultura marina
Keywords: European sea bass
Gill morphology
Oxidative stress
Functional diets
Prebiotics, et al
Issue Date: 2021
Project: Genomic and nutritional innovations for genetically superior farmed fish to improve efficiency in European aquaculture 
Dieta Funcional Para Sustitución de Ingredientes Marinos en Peces: Maximización de Resistencia A Patogenos Via Refuerzo de Barreras Epiteliales y Herramientas de Inmunización 
Journal: Frontiers in Immunology 
Abstract: An effective replacement for fish meal (FM) and fish oil (FO) based on plant-based raw materials in the feed of marine fish species is necessary for the sustainability of the aquaculture sector. However, the use of plant-based raw materials to replace FM and FO has been associated with several negative health effects, some of which are related to oxidative stress processes that can induce functional and morphological alterations in mucosal tissues. This study aimed to evaluate the effects of dietary oligosaccharides of plant origin (5,000 ppm; galactomannan oligosaccharides, GMOS) and a phytogenic feed additive (200 ppm; garlic oil and labiatae plant extract mixture, PHYTO) on the oxidative stress status and mucosal health of the gills of juvenile European sea bass (Dicentrarchus labrax). The experimental diets, low FM and FO diets (10%FM/6%FO) were supplemented with GMOS from plant origin and PHYTO for 63 days. GMOS and PHYTO did not significantly affect feed utilization, fish growth, and survival. GMOS and PHYTO downregulated the expression of β-act, sod, gpx, cat, and gr in the gills of the fish compared with that in fish fed the control diet. The expression of hsp70 and ocln was upregulated and downregulated, respectively, in the GMOS group compared with that in the control group, whereas the expression of zo-1 was downregulated in the PHYTO group compared with that in the GMOS group. The morphological, histopathological, immunohistochemical, and biochemical parameters of the fish gills were mostly unaffected by GMOS and PHYTO. However, the PHYTO group had lower incidence of lamellar fusion than did the control group after 63 days. Although the tissular distribution of goblet cells was unaffected by GMOS and PHYTO, goblet cell size showed a decreasing trend (−11%) in the GMOS group. GMOS and PHYTO significantly reduced the concentration of PCNA+ in the epithelium of the gills. The above findings indicated that GMOS and PHYTO in low FM/FO-based diets protected the gill epithelia of D. labrax from oxidative stress by modulating the expression of oxidative enzyme-related genes and reducing the density of PCNA+ cells in the gills of the fish.
ISSN: 1664-3224
DOI: 10.3389/fimmu.2021.663106
Source: Frontiers in Immunology [ISSN 1664-3224], v. 12, 663106, (Mayo 2021)
Appears in Collections:Artículos
Adobe PDF (9,43 MB)
Show full item record


checked on Mar 26, 2023

Page view(s)

checked on Mar 4, 2023


checked on Mar 4, 2023

Google ScholarTM




Export metadata

Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.