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Title: Low-Cost Hyperspectral Push-broom Microscope, targeting Smart Farming Applications
Authors: Ortega Sarmiento, Samuel 
Guerra Hernández, Raúl Celestino 
Fabelo Gómez, Himar Antonio 
Díaz Martín, María 
López Suárez, Sebastián 
Marrero Callicó, Gustavo Iván 
Sarmiento Rodríguez, Roberto 
UNESCO Clasification: 220990 Tratamiento digital. Imágenes
Keywords: Hyperspectral imaging
Microscopic analysis
Push-broom camera
Issue Date: 2019
Project: Identificación Hiperespectral de Tumores Cerebrales (Ithaca) 
Plataforma H2/Sw Distribuida Para El Procesamiento Inteligente de Información Sensorial Heterogenea en Aplicaciones de Supervisión de Grandes Espacios Naturales 
Conference: 34th Conference on Design of Circuits and Integrated Systems, DCIS 2019 
Abstract: Currently, the use of hyperspectral imaging (HSI) for the inspection of microscopic samples in an emerging trend in different fields. The use of push-broom hyperspectral (HS) cameras against other HSI technologies is motivated by their high spectral resolution and their capabilities to exploit spectral ranges beyond 1000 nm. Nevertheless, the use of push-broom cameras coupled to microscopes imposes to perform an accurate spatial scanning of the sample to collect the HS data. In this manuscript, we present a methodology to correctly set-up a HS push-broom microscope to acquire high-quality HS images. First, we present a custom 3D printed mechanical system developed to perform the spatial scanning by performing a linear movement that controls the microscope stage. Then, we discuss the most important characteristics of a microscopic HS system and we propose an image analysis method able to quantitatively verify the correct setup of the system prior to acquire high-quality HS images. Finally, we present a set of images acquired from real microscopic samples, showing the potential of HSI for the microscopic analysis.
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