Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/106721
Title: Ionic gold demonstrates antimicrobial activity against Pseudomonas aeruginosa strains due to cellular ultrastructure damage
Authors: Reyes Torres, Miguel
Slate, Anthony J.
Ryder, Steven F.
Akram, Maliha
Carrascosa Iruzubieta, Conrado Javier 
Whitehead, Kathryn A.
UNESCO Clasification: 310905 Microbiología
Keywords: Antimicrobials
Ionic Gold
Mechanism Of Action
Metals
Pseudomonas Aeruginosa, et al
Issue Date: 2021
Journal: Archives of Microbiology 
Abstract: Due to the ever-increasing rise of antimicrobial resistant (AMR) bacteria, the development of alternative antimicrobial agents is a global priority. The antimicrobial activity of ionic gold was explored against four Pseudomonas aeruginosa strains with different AMR profiles in order to determine the antimicrobial activity of ionic gold and elucidate the mechanisms of action. Disc diffusion assays (zone of inhibition: ZoI) coupled with minimum inhibitory/bactericidal concentrations (MIC/MBC) were conducted to determine the antimicrobial efficacy of ionic gold. Scanning electron microscopy (SEM) was used to visualise morphological changes to the bacterial cell ultrastructure. Strains with increased AMR were slower to grow which is likely a fitness cost due to the enhanced AMR activity. Although greater concentrations of ionic gold were required to promote antimicrobial activity, ionic gold demonstrated similar antimicrobial values against all strains tested. Lowry assay results indicated that protein leakage was apparent following incubation with ionic gold, whilst SEM revealed cellular ultrastructure damage. This study suggests that the application of ionic gold as an alternative antimicrobial is promising, particularly against AMR P. aeruginosa. The antimicrobial activity of ionic gold against P. aeruginosa could potentially be utilised as an alternative therapeutic option in wound management, an approach that could benefit healthcare systems worldwide.
URI: http://hdl.handle.net/10553/106721
ISSN: 0302-8933
DOI: 10.1007/s00203-021-02270-1
Source: Archives of Microbiology [ISSN 0302-8933], (Enero 2021)
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