Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/106588
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dc.contributor.authorKlionsky, Daniel J.en_US
dc.contributor.authorAbdel-Aziz, Amal Kamalen_US
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dc.contributor.authorTong, Xinen_US
dc.contributor.authorTooze, Sharon A.en_US
dc.contributor.authorTorgersen, Maria L.en_US
dc.contributor.authorTorii, Satoruen_US
dc.contributor.authorTowers, Christina G.en_US
dc.contributor.authorTowns, Robertoen_US
dc.contributor.authorToyokuni, Shinyaen_US
dc.contributor.authorTrajkovic, Vladimiren_US
dc.contributor.authorTramontano, Donatellaen_US
dc.contributor.authorTravassos, Leonardo H.en_US
dc.contributor.authorTrelford, Charles B.en_US
dc.contributor.authorTremel, Shirleyen_US
dc.contributor.authorTrougakos, Ioannis P.en_US
dc.contributor.authorTschan, Mario P.en_US
dc.contributor.authorTse, Hung-Faten_US
dc.contributor.authorTse, Tak Fuen_US
dc.contributor.authorTsugawa, Hitoshien_US
dc.contributor.authorTsvetkov, Andrey S.en_US
dc.contributor.authorTumbarello, David A.en_US
dc.contributor.authorTumtas, Yasinen_US
dc.contributor.authorTuñón, María J.en_US
dc.contributor.authorTurcotte, Sandraen_US
dc.contributor.authorTurk, Borisen_US
dc.contributor.authorTurk, Vitoen_US
dc.contributor.authorTurner, Bradley J.en_US
dc.contributor.authorTuxworth, Richard I.en_US
dc.contributor.authorTyler, Jessica K.en_US
dc.contributor.authorTyutereva, Elena V.en_US
dc.contributor.authorUgun-Klusek, Aslihanen_US
dc.contributor.authorUhlig, Holm H.en_US
dc.contributor.authorUłamek-Kozioł, Marzenaen_US
dc.contributor.authorUlasov, Ilya V.en_US
dc.contributor.authorUmekawa, Midorien_US
dc.contributor.authorUngermann, Christianen_US
dc.contributor.authorUnno, Reien_US
dc.contributor.authorUrbe, Sylvieen_US
dc.contributor.authorUribe-Carretero, Elisabeten_US
dc.contributor.authorÜstün, Suayiben_US
dc.contributor.authorUversky, Vladimir Nen_US
dc.contributor.authorVaccari, Thomasen_US
dc.contributor.authorVaccaro, Maria I.en_US
dc.contributor.authorVakifahmetoglu-Norberg, Helinen_US
dc.contributor.authorValdor, Ruten_US
dc.contributor.authorValko, Ayelénen_US
dc.contributor.authorValverde, Angela M.en_US
dc.contributor.authorvan der Veen, Stijnen_US
dc.contributor.authorVan Kaer, Lucen_US
dc.contributor.authorvan Loosdregt, Jorgen_US
dc.contributor.authorvan Wijk, Sjoerd J.L.en_US
dc.contributor.authorVandenberghe, Wimen_US
dc.contributor.authorVanhorebeek, Ilseen_US
dc.contributor.authorVannier-Santos, Marcos A.en_US
dc.contributor.authorVannini, Nicolaen_US
dc.contributor.authorVanrell, M. Cristinaen_US
dc.contributor.authorVantaggiato, Chiaraen_US
dc.contributor.authorVarano, Gabrieleen_US
dc.contributor.authorVarela-Nieto, Isabelen_US
dc.contributor.authorVarga, Mátéen_US
dc.contributor.authorVasconcelos, M. Helenaen_US
dc.contributor.authorVats, Somyaen_US
dc.contributor.authorVavvas, Demetrios G.en_US
dc.contributor.authorVega-Naredo, Ignacioen_US
dc.contributor.authorVega-Rubin-de-Celis, Silviaen_US
dc.contributor.authorVelasco, Guillermoen_US
dc.contributor.authorVellai, Tiboren_US
dc.contributor.authorVelotti, Francescaen_US
dc.contributor.authorVerdier, Mireilleen_US
dc.contributor.authorVerginis, Panayotisen_US
dc.contributor.authorVergne, Isabelleen_US
dc.contributor.authorVerstreken, Patriken_US
dc.contributor.authorVervliet, Timen_US
dc.contributor.authorVervoorts, Jörgen_US
dc.contributor.authorVessoni, Alexandre T.en_US
dc.contributor.authorVictor, Victor M.en_US
dc.contributor.authorVidal, Michelen_US
dc.contributor.authorVierstra, Richard D.en_US
dc.contributor.authorViganó, Soniaen_US
dc.contributor.authorVihinen, Helenaen_US
dc.contributor.authorVijayan, Vinoyen_US
dc.contributor.authorVila, Miquelen_US
dc.contributor.authorVilar, Marçalen_US
dc.contributor.authorVillarejo-Zori, Beatrizen_US
dc.contributor.authorVillarroya, Francescen_US
dc.contributor.authorVincent, Olivieren_US
dc.contributor.authorVindis, Cecileen_US
dc.contributor.authorVisnjic, Doraen_US
dc.contributor.authorVoitsekhovskaja, Olga V.en_US
dc.contributor.authorVolonté, Cinziaen_US
dc.contributor.authorVolta, Mattiaen_US
dc.contributor.authorVomero, Martaen_US
dc.contributor.authorVon Haefen, Clarissaen_US
dc.contributor.authorVooijs, Marc A.en_US
dc.contributor.authorVoos, Wolfgangen_US
dc.contributor.authorVucicevic, Ljubicaen_US
dc.contributor.authorWade-Martins, Richarden_US
dc.contributor.authorWaguri, Satoshien_US
dc.contributor.authorWaite, Kenrick A.en_US
dc.contributor.authorWalker, David W.en_US
dc.contributor.authorWalker, Mark J.en_US
dc.contributor.authorWalker, Simon A.en_US
dc.contributor.authorWalter, Jochenen_US
dc.contributor.authorWang, Boen_US
dc.contributor.authorWang, Chao-Yungen_US
dc.contributor.authorWang, Chenen_US
dc.contributor.authorWang, Chenweien_US
dc.contributor.authorWang, Cun-Yuen_US
dc.contributor.authorWang, Dongen_US
dc.contributor.authorWang, Fangyangen_US
dc.contributor.authorWang, Fengen_US
dc.contributor.authorWang, Guansongen_US
dc.contributor.authorWang, Hanen_US
dc.contributor.authorWang, Haoen_US
dc.contributor.authorWang, Hexiangen_US
dc.contributor.authorWang, Jigangen_US
dc.contributor.authorWang, Jiouen_US
dc.contributor.authorWang, Jundongen_US
dc.contributor.authorWang, Kuien_US
dc.contributor.authorWang, Maggie Haitianen_US
dc.contributor.authorWang, Meiqingen_US
dc.contributor.authorWang, Nanbuen_US
dc.contributor.authorWang, Pengweien_US
dc.contributor.authorWang, Pingen_US
dc.contributor.authorWang, Pingen_US
dc.contributor.authorWang, Qingen_US
dc.contributor.authorWang, Qiong A.en_US
dc.contributor.authorWang, Wen-Taoen_US
dc.contributor.authorWang, Xinnanen_US
dc.contributor.authorWang, Xuejunen_US
dc.contributor.authorWang, Yanen_US
dc.contributor.authorWang, Yanchangen_US
dc.contributor.authorWang, Yanzhuangen_US
dc.contributor.authorWang, Yihuaen_US
dc.contributor.authorWang, Yuen_US
dc.contributor.authorWang, Yuqien_US
dc.contributor.authorWang, Zheen_US
dc.contributor.authorWang, Zhenyuen_US
dc.contributor.authorWang, Zhouguangen_US
dc.contributor.authorWarnes, Garyen_US
dc.contributor.authorWatada, Hirotakaen_US
dc.contributor.authorWatanabe, Eizoen_US
dc.contributor.authorWatchon, Maxinneen_US
dc.contributor.authorWawrzyńska, Annaen_US
dc.contributor.authorWeaver, Timothy E.en_US
dc.contributor.authorWegrzyn, Grzegorzen_US
dc.contributor.authorWehman, Ann M.en_US
dc.contributor.authorWei, Huafengen_US
dc.contributor.authorWei, Leien_US
dc.contributor.authorWei, Taotaoen_US
dc.contributor.authorWeiergräber, Oliver H.en_US
dc.contributor.authorWeihl, Conrad C.en_US
dc.contributor.authorWeindl, Güntheren_US
dc.contributor.authorWeiskirchen, Ralfen_US
dc.contributor.authorWells, Alanen_US
dc.contributor.authorWen, Xinen_US
dc.contributor.authorWerner, Antoniaen_US
dc.contributor.authorWeykopf, Beatriceen_US
dc.contributor.authorWhitton, J. Lindsayen_US
dc.contributor.authorWhitworth, Alexander J.en_US
dc.contributor.authorWiktorska, Katarzynaen_US
dc.contributor.authorWildenberg, Manon E.en_US
dc.contributor.authorWileman, Tomen_US
dc.contributor.authorWilliams, Bretten_US
dc.contributor.authorWilliams, Robin S.B.en_US
dc.contributor.authorWilliams, Roger L.en_US
dc.contributor.authorWilson, Richard A.en_US
dc.contributor.authorWinner, Beateen_US
dc.contributor.authorWitkin, Steven S.en_US
dc.contributor.authorWong, Estheren_US
dc.contributor.authorWong, Jack Hoen_US
dc.contributor.authorWong, Richard W.en_US
dc.contributor.authorWong, Vincent Kam Waien_US
dc.contributor.authorWong, W. Wei-Lynnen_US
dc.contributor.authorWu, An-Guoen_US
dc.contributor.authorWu, Chengbiaoen_US
dc.contributor.authorWu, Jianen_US
dc.contributor.authorWu, Junfangen_US
dc.contributor.authorWu, Minen_US
dc.contributor.authorWu, Shan-Yingen_US
dc.contributor.authorWu, Shengzhouen_US
dc.contributor.authorWu, William K.K.en_US
dc.contributor.authorWu, Xiaohongen_US
dc.contributor.authorWu, Xiaoqingen_US
dc.contributor.authorWu, Yao-Wenen_US
dc.contributor.authorWu, Yihuaen_US
dc.contributor.authorXavier, Ramnik J.en_US
dc.contributor.authorXia, Lixinen_US
dc.contributor.authorXiang, Jinen_US
dc.contributor.authorXiang, Mingliangen_US
dc.contributor.authorXiao, Guozhien_US
dc.contributor.authorXiao, Hengyien_US
dc.contributor.authorXiao, Hong-taoen_US
dc.contributor.authorXiao, Lanen_US
dc.contributor.authorXiao, Shien_US
dc.contributor.authorXiao, Yinen_US
dc.contributor.authorXie, Baomingen_US
dc.contributor.authorXie, Minen_US
dc.contributor.authorXie, Yuxiangen_US
dc.contributor.authorXie, Zhonglinen_US
dc.contributor.authorXu, Enen_US
dc.contributor.authorXu, Haoxingen_US
dc.contributor.authorXu, JinRongen_US
dc.contributor.authorXu, Liangen_US
dc.contributor.authorXu, Wen Wenen_US
dc.contributor.authorXue, Yuen_US
dc.contributor.authorYamaguchi, Masamitsuen_US
dc.contributor.authorYamaguchi, Osamuen_US
dc.contributor.authorYamamoto, Aien_US
dc.contributor.authorYan, Zhenen_US
dc.contributor.authorYanagi, Yasuoen_US
dc.contributor.authorYang, Dun-Shengen_US
dc.contributor.authorYang, Huanen_US
dc.contributor.authorYang, Huang-Tianen_US
dc.contributor.authorYang, Jin-Mingen_US
dc.contributor.authorYang, Jingyuen_US
dc.contributor.authorYang, Seungwonen_US
dc.contributor.authorYang, Shuen_US
dc.contributor.authorYang, Shun-Faen_US
dc.contributor.authorYang, Wannianen_US
dc.contributor.authorYang, Wei Yuanen_US
dc.contributor.authorYang, Xiaoyongen_US
dc.contributor.authorYang, Yingen_US
dc.contributor.authorYao, Honghongen_US
dc.contributor.authorYao, Xiaoqiangen_US
dc.contributor.authorYao, Yong-Mingen_US
dc.contributor.authorYasui, Takahiroen_US
dc.contributor.authorYazdankhah, Meysamen_US
dc.contributor.authorYen, Paul M.en_US
dc.contributor.authorYin, Xiao-Mingen_US
dc.contributor.authorYin, Yanhaien_US
dc.contributor.authorYing, Zhengen_US
dc.contributor.authorYip, Calvin K.en_US
dc.contributor.authorYoo, Young H.en_US
dc.contributor.authorYu, Boxuanen_US
dc.contributor.authorYu, Haiyangen_US
dc.contributor.authorYu, Junen_US
dc.contributor.authorYu, Ming-Lungen_US
dc.contributor.authorYu, Zhouen_US
dc.contributor.authorYuan, Junyingen_US
dc.contributor.authorYuan, Shyng-Shiou F.en_US
dc.contributor.authorYuan, Yanggangen_US
dc.contributor.authorYuan, Zengqiangen_US
dc.date.accessioned2021-04-07T07:35:51Z-
dc.date.available2021-04-07T07:35:51Z-
dc.date.issued2021en_US
dc.identifier.issn1554-8627en_US
dc.identifier.urihttp://hdl.handle.net/10553/106588-
dc.description.abstractIn 2008, we published the first set of guidelines for standardizing research in autophagy. Since then, this topic has received increasing attention, and many scientists have entered the field. Our knowledge base and relevant new technologies have also been expanding. Thus, it is important to formulate on a regular basis updated guidelines for monitoring autophagy in different organisms. Despite numerous reviews, there continues to be confusion regarding acceptable methods to evaluate autophagy, especially in multicellular eukaryotes. Here, we present a set of guidelines for investigators to select and interpret methods to examine autophagy and related processes, and for reviewers to provide realistic and reasonable critiques of reports that are focused on these processes. These guidelines are not meant to be a dogmatic set of rules, because the appropriateness of any assay largely depends on the question being asked and the system being used. Moreover, no individual assay is perfect for every situation, calling for the use of multiple techniques to properly monitor autophagy in each experimental setting. Finally, several core components of the autophagy machinery have been implicated in distinct autophagic processes (canonical and noncanonical autophagy), implying that genetic approaches to block autophagy should rely on targeting two or more autophagy-related genes that ideally participate in distinct steps of the pathway. Along similar lines, because multiple proteins involved in autophagy also regulate other cellular pathways including apoptosis, not all of them can be used as a specific marker for bona fide autophagic responses. Here, we critically discuss current methods of assessing autophagy and the information they can, or cannot, provide. Our ultimate goal is to encourage intellectual and technical innovation in the field.en_US
dc.languageengen_US
dc.relation.ispartofAutophagyen_US
dc.sourceAutophagy [ISSN 1554-8627], v. 17 (1)en_US
dc.subject3206 Ciencias de la nutriciónen_US
dc.subject.otherAutophagosomeen_US
dc.subject.otherCanceren_US
dc.subject.otherFluxen_US
dc.subject.otherLC3en_US
dc.subject.otherLysosomeen_US
dc.subject.otherMacroautophagyen_US
dc.subject.otherNeurodegenerationen_US
dc.subject.otherPhagophoreen_US
dc.subject.otherStressen_US
dc.subject.otherVacuoleen_US
dc.titleGuidelines for the use and interpretation of assays for monitoring autophagy (4th edition)en_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.identifier.doi10.1080/15548627.2020.1797280en_US
dc.identifier.issue1-
dc.relation.volume17en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgcen_US
dc.description.sjr3,259
dc.description.jcr13,391
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
dc.description.miaricds10,7
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR IUIBS: Rendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Educación Física-
crisitem.author.deptDepartamento de Filología Moderna, Traducción e Interpretación-
crisitem.author.deptGIR IUIBS: Rendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Educación Física-
crisitem.author.deptGIR IUIBS: Rendimiento humano, ejercicio físico y salud-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Educación Física-
crisitem.author.orcid0000-0002-9215-6234-
crisitem.author.orcid0000-0002-3685-2331-
crisitem.author.orcid0000-0001-8463-397X-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameLópez Calbet, José Antonio-
crisitem.author.fullNamePayet, Karine Marie Muriel-
crisitem.author.fullNameMartín Rincón, Marcos-
crisitem.author.fullNameNavas Martínez,Ana Belen-
crisitem.author.fullNameMorales Álamo, David-
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