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Title: Pathological and Immunohistochemical Studies of Experimental Mycoplasma pneumoniae in Gerbils (Meriones unguiculatus)
Authors: Rodríguez Guisado, Francisco 
Ramírez Corbera, Ana Sofía 
Castro Alonso, Pedro Luis 
Poveda Guerrero, José Bismarck 
UNESCO Clasification: 310907 Patología
310903 Inmunología
Keywords: Bronchus associated lymphoid tissue
Mycoplasma pneumoniae
Issue Date: 2021
Journal: Journal of Comparative Pathology 
Abstract: Mycoplasma pneumoniae (Mp) is a leading cause of human community-acquired pneumonia. To investigate the pathogenesis of the infection, 36 gerbils were intranasally inoculated with Mp culture (30 animals) or sterile mycoplasma broth (6 animals) and euthanized from 1 to 5 weeks post infection. A morphological and immunohistochemical study was carried out in all animals to determine the cellular populations present in lung parenchyma. Polyclonal and monoclonal antibodies were used to detect antigens of Mp and CD3, CD4, CD8 and CD79 lymphocytes, as well as cells containing S100 and major histocompatibility complex class II (MHC-II) antigens. There was progressive infiltration of mononuclear cells in the lamina propria of bronchi and bronchioles, and hyperplasia of the bronchus-associated lymphoid tissue (BALT) in the infected animals. BALT contained dendritic cells immunopositive to S100 and MHC-II and numerous CD3, CD4 and CD79 lymphocytes. The immunohistochemical results showed that T lymphocytes, particularly CD4 and CD79 cells, may play a role in the immune response of gerbils against Mp. This experimental model is valuable for investigation of the pathogenesis of Mp infection and may assist in the development of therapeutic strategies.
ISSN: 0021-9975
DOI: 10.1016/j.jcpa.2021.01.011
Source: Journal of Comparative Pathology [ISSN 0021-9975], v. 184, p. 37-43
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