Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/77928
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dc.contributor.authorKusebauch, Ulrikeen_US
dc.contributor.authorHernández Castellano, Lorenzo Enriqueen_US
dc.contributor.authorBislev, Stine L.en_US
dc.contributor.authorMoritz, Robert L.en_US
dc.contributor.authorRøntved, Christine M.en_US
dc.contributor.authorBendixen, Emøkeen_US
dc.date.accessioned2021-03-04T13:09:12Z-
dc.date.available2021-03-04T13:09:12Z-
dc.date.issued2018en_US
dc.identifier.issn0022-0302en_US
dc.identifier.urihttp://hdl.handle.net/10553/77928-
dc.description.abstractMastitis is a major challenge to bovine health. The detection of sensitive markers for mastitis in dairy herds is of great demand. Suitable biomarkers should be measurable in milk and should report pathogen-specific changes at an early stage to support earlier diagnosis and more efficient treatment. However, the identification of sensitive biomarkers in milk has remained a challenge, in part due to their relatively low concentration in milk. In the present study, we used a selected reaction monitoring (SRM) mass spectrometry approach, which allowed the absolute quantitation of 13 host response proteins in milk for the first time. These proteins were measured over a 54-h period upon an in vivo challenge with cell wall components from either gram-negative (lipopolysaccharide from Escherichia coli; LPS) or gram-positive bacteria (peptidoglycan from Staphylococcus aureus; PGN). Whereas our data clearly demonstrate that all challenged animals have consistent upregulation of innate immune response proteins after both LPS and PGN challenge, the data also reveal clearly that LPS challenge unleashes faster and shows a more intense host response compared with PGN challenge. Biomarker candidates that may distinguish between gram-negative and gram-positive bacteria include α-2 macroglobulin, α-1 antitrypsin, haptoglobin, serum amyloid A3, cluster of differentiation 14, calgranulin B, cathepsin C, vanin-1, galectin 1, galectin 3, and IL-8. Our approach can support further studies of large cohorts of animals with natural occurring mastitis, to validate the relevance of these suggested biomarkers in dairy production.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Dairy Scienceen_US
dc.sourceJournal of Dairy Science [ISSN 0022-0302], v. 101(7), p. 6532-6541en_US
dc.subject3104 Producción Animalen_US
dc.subject.otherMastitisen_US
dc.subject.otherSelected reaction monitoringen_US
dc.subject.otherHost response proteinsen_US
dc.subject.otherLipopolysaccharideen_US
dc.subject.otherPeptidoglycanen_US
dc.titleSelected reaction monitoring mass spectrometry of mastitis milk reveals pathogen-specific regulation of bovine host response proteinsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typearticleen_US
dc.identifier.doi10.3168/jds.2017-14312en_US
dc.identifier.issue7-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgcNoen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.sjr1,34
dc.description.jcr3,082
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.orcid0000-0003-2729-0434-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameHernández Castellano, Lorenzo Enrique-
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