Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/77925
DC FieldValueLanguage
dc.contributor.authorValent, Danielen_US
dc.contributor.authorYeste, Nataliaen_US
dc.contributor.authorHernández Castellano, Lorenzo Enriqueen_US
dc.contributor.authorArroyo, Lauraen_US
dc.contributor.authorWu, Weien_US
dc.contributor.authorGarcía-Contreras, Consolaciónen_US
dc.contributor.authorVázquez-Gómez, Martaen_US
dc.contributor.authorGonzález-Bulnes, Antonioen_US
dc.contributor.authorBendixen, Emøkeen_US
dc.contributor.authorBassols, Annaen_US
dc.date.accessioned2021-03-04T13:06:16Z-
dc.date.available2021-03-04T13:06:16Z-
dc.date.issued2019en_US
dc.identifier.issn1874-3919en_US
dc.identifier.urihttp://hdl.handle.net/10553/77925-
dc.description.abstractIntrauterine growth restriction (IUGR) is characterized by reduced growth and weight of the foetus, mainly due to the lack of nutrients and oxygen. Animals affected by IUGR show changes in specific brain areas and several neuronal processes. Female offspring affected by IUGR show increased survival and development compared to males. The objective of this study was to analyse changes in the hippocampus proteome in male and female piglets affected by IUGR. Seven pregnant Iberian sows were fed from Day 35 of pregnancy onwards at 50% of their requirements. At Day 100 of pregnancy, foetuses were obtained and classified by sex and weight, as mild IUGR (Normal Body Weight) versus severe IUGR (Low Body Weight). Hippocampi were dissected and the proteomes analysed by SWATH-MS DIA. In this study, 1497 proteins were identified of which 260 were quantitatively analysed. All differential proteins were more abundant in females versus males and were involved in protein synthesis, neuronal development, metabolism, antiapoptotic signalling and vesicular transport. Our findings support that female foetuses tolerate nutrient limitation better than males, especially under mild IUGR. Under severe IUGR, females still seems to maintain normal lipid metabolism and antiapoptotic signalling, which may be related to the increased female survival. SIGNIFICANCE: In the last years, proteomics have been used to evidence differences related to sex in non-reproductive organs. Intrauterine Growth Restriction (IUGR) can affect female and male offspring differently. Female offspring has stronger protective strategies compared to males, enhancing growth and postnatal survival. Most studies regarding this issue have focused on metabolic organs (i.e. liver). However, the predominance of neurodevelopmental disorders in males suggests that the central nervous system in female offspring adapt better to nutritional stress conditions than that of males. Based on the differential protein expression in hippocampal samples, our work demonstrates that female foetuses indeed adapt better to IUGR than males, especially under mild IUGR conditions. In severe IUGR conditions, differences between males and females were not so evident, but even in this case, the remaining differences suggest increased survival in females than in males.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Proteomicsen_US
dc.sourceJournal of Proteomics [ISSN 1874-3919], n. 204en_US
dc.subject3104 Producción Animalen_US
dc.subject.otherHippocampusen_US
dc.subject.otherIntrauterine growth restrictionen_US
dc.subject.otherPigen_US
dc.subject.otherProteomicsen_US
dc.subject.otherSWATH-MSen_US
dc.subject.otherSexen_US
dc.titleSWATH-MS quantitative proteomic investigation of intrauterine growth restriction in a porcine model reveals sex differences in hippocampus developmenten_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typearticleen_US
dc.identifier.doi10.1016/j.jprot.2019.103391en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgcNoen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.sjr1,189
dc.description.jcr3,509
dc.description.sjrqQ1
dc.description.jcrqQ2
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR Producción y Biotecnología Animal-
crisitem.author.deptIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.orcid0000-0003-2729-0434-
crisitem.author.parentorgIU de Sanidad Animal y Seguridad Alimentaria-
crisitem.author.fullNameHernández Castellano, Lorenzo Enrique-
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