Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/7757
Title: Behavior of respiratory metabolism and pyridine nucleotide levels in Oxyrrhis marina during starvation
Authors: Osma, Natalia 
Director: Packard, Theodore T. 
Gómez, May 
UNESCO Clasification: 251001 Oceanografía biológica
Keywords: Respiration
ETS activity
pyridine nucleotides
NADt and NADPt
Oxyrrhis marina
Issue Date: 2010
Project: Estudio de Un Nuevo Modelo Mecanistico Para El Metabolismo Del Zooplancton 
Abstract: Respiration in eukaryotes is a physiological process that occurs wherever the oxygen and organic matter are present. Respiration is oxygen consumption (RO2) catalyzed by the respiratory electron transport system (ETS) enzymes. The physiological RO2 rate depends directly on the enzymatic control of the respiratory ETS activity. According to Packard et al. (1996), we argue that substrate availability is the most probable regulatory mechanism controlling this activity. The major ETS substrates are the pyridine nucleotides (PNs) and they occur as nicotine adenine dinucleotide (NAD) and as nicotine adenine dinucleotide phosphate (NADP). Up to now, no studies have quantified their intracellular concentrations in a marine dinoflagellate. Oxyrrhis marina is a microheterotrophic marine dinoflagellate which has been widely investigated. Nevertheless, no study has characterized its respiratory metabolism from measurements of oxygen consumption (RO2) and ETS activity. For this reason, our main objectives were 1) to measure RO2 and ETS activity in O. marina and to determine their behavior during starvation, and 2) to quantify the PN levels during the same period. To achieve these objectives, we fed O. marina ad libitum and then we monitored the ETS, RO2, protein and PN levels for 17 days.
Faculty: Facultad de Ciencias del Mar
URI: http://hdl.handle.net/10553/7757
Rights: by-nc-nd
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