Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/77266
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dc.contributor.authorPérez Barreto,Daviniaen_US
dc.contributor.authorMuñoz-Caro, T.en_US
dc.contributor.authorSilva, L. M.R.en_US
dc.contributor.authorMuñoz Ojeda, María Del Carmenen_US
dc.contributor.authorMolina Caballero, José Manuelen_US
dc.contributor.authorTaubert, A.en_US
dc.contributor.authorHermosilla, C.en_US
dc.contributor.authorRuiz Reyes, Antonioen_US
dc.date.accessioned2021-01-20T11:11:54Z-
dc.date.available2021-01-20T11:11:54Z-
dc.date.issued2021en_US
dc.identifier.issn0014-4894en_US
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/77266-
dc.description.abstractEimeria ninakohlyakimovae represents a highly pathogenic coccidian parasite causing severe haemorrhagic typhlocolitis in goat kids worldwide. NETosis was recently described as an efficient defense mechanism of polymorphonuclear neutrophils (PMN) acting against different parasites in vitro and in vivo. In vitro interactions of caprine PMN with parasitic stages of E. ninakohlyakimovae (i. e. oocysts and sporozoites) as well as soluble oocyst antigens (SOA) were analyzed at different ratios, concentrations and time spans. Extracellular DNA staining was used to illustrate classical molecules induced during caprine NETosis [i. e. histones (H3) and neutrophil elastase (NE)] via antibody-based immunofluorescence analyses. Functional inhibitor treatments with DPI and DNase I were applied to unveil role of NADPH oxidase (NOX) and characterize DNA-backbone composition of E. ninakohlyakimovae-triggered caprine NETosis. Scanning electron microscopy (SEM)- and immunofluorescence-analyses demonstrated that caprine PMN underwent NETosis upon contact with sporozoites and oocysts of E. ninakohlyakimovae, ensnaring filaments which firmly entrapped parasites. Detailed co-localization studies of E. ninakohlyakimovae-induced caprine NETosis revealed presence of PMN-derived DNA being adorned with nuclear H3 and NE corroborating molecular characteristics of NETosis. E. ninakohlyakoimovae-induced caprine NETosis was found to be NOX-independent since DPI inhibition led to a slight decrease of NETosis. Exposure of caprine PMN to vital E. ninakohlyakimovae sporozoites as well as SOA resulted in up-regulation of IL-12, TNF-α, IL-6, CCL2 and iNOS gene transcription in stimulated PMN. Since vital E. ninakohlyakimovae-sporozoites induced caprine NETosis, this effective entrapment mechanism might reduce initial sporozoite epithelial host cell invasion during goat coccidiosis ultimately resulting in less macromeront formation and reduced merozoites I production.en_US
dc.languageengen_US
dc.relation.ispartofExperimental parasitologyen_US
dc.sourceExperimental Parasitology [ISSN 0014-4894], n. 220, (Enero 2021)en_US
dc.subject310907 Patologíaen_US
dc.subject.otherChemokinesen_US
dc.subject.otherEimeria Ninakohlyakimovaeen_US
dc.subject.otherGoatsen_US
dc.subject.otherNetosisen_US
dc.subject.otherPmnen_US
dc.titleEimeria ninakohlyakimovae casts NOX-independent NETosis and induces enhanced IL-12, TNF-α, IL-6, CCL2 and iNOS gene transcription in caprine PMNen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1016/j.exppara.2020.108034en_US
dc.identifier.scopus85096541115-
dc.contributor.authorscopusid36096860400-
dc.contributor.authorscopusid56182418900-
dc.contributor.authorscopusid55791642600-
dc.contributor.authorscopusid57199669332-
dc.contributor.authorscopusid57218216797-
dc.contributor.authorscopusid6603706756-
dc.contributor.authorscopusid6602143835-
dc.contributor.authorscopusid57201387360-
dc.identifier.eissn1090-2449-
dc.relation.volume220en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.date.coverdateEnero 2021en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.sjr0,494
dc.description.jcr2,132
dc.description.sjrqQ3
dc.description.jcrqQ3
dc.description.scieSCIE
dc.description.miaricds11,0
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0002-0203-5034-
crisitem.author.orcid0000-0002-8338-2512-
crisitem.author.orcid0000-0003-0668-5496-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.fullNamePérez Barreto,Davinia-
crisitem.author.fullNameMuñoz Ojeda, María Del Carmen-
crisitem.author.fullNameMolina Caballero, José Manuel-
crisitem.author.fullNameRuiz Reyes, Antonio-
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