Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/76680
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dc.contributor.authorRodríguez Navarro, José Luisen_US
dc.contributor.authorErmel, Richard W.en_US
dc.contributor.authorKenny, Thomas P.en_US
dc.contributor.authorBrooks, Dale L.en_US
dc.contributor.authorDaMassa, Al J.en_US
dc.date.accessioned2020-12-15T11:33:07Z-
dc.date.available2020-12-15T11:33:07Z-
dc.date.issued1997en_US
dc.identifier.issn1040-6387en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/76680-
dc.description.abstractA specific diagnostic method using the polymerase chain reaction, together with restriction endonuclease digestion patterns, was developed for members of the ''Mycoplasma mycoides cluster'' that normally occur in the United States (i.e., Mycoplasma mycoides subsp. mycoides Large Colony and Mycoplasma capricolum subsp. capricolum in addition to ''cluster'' mycoplasma, bovine serogroup 7, and Mycoplasma putrefaciens. The digestion of ''cluster'' polymerase chain reaction DNA (1,225 bp) amplification products with restriction enzymes AseI and SspI gave mycoplasma species-specific patterns for ail strains of M. mycoides subsp. mycoides Large Colony, M. capricolum subsp. capricolum, and bovine group 7 tested. Moreover, we found a nonspecific amplification product for M. putrefaciens that occurred with the oligonucleotide primers used for the ''M. mycoides cluster'' reaction. However, the restriction endonuclease digestion patterns observed with the restriction enzymes AluI, AseI, and SspI for M. putrefaciens were different than the digestion patterns obtained for the other ''cluster'' mycoplasmas. This report confirms the usefulness of polymerase chain reaction DNA amplification allied with restriction enzyme digestion profile analysis for the rapid and specific identification of mycoplasmas belonging to the ''M. mycoides cluster'' and M. putrefaciens.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Veterinary Diagnostic Investigationen_US
dc.sourceJournal of Veterinary Diagnostic Investigation [ISSN 1040-6387], v. 9 (2), p. 186-190, (Abril 1997)en_US
dc.subject320505 Enfermedades infecciosasen_US
dc.subject310907 Patologíaen_US
dc.subject.otherDNA Probeen_US
dc.subject.otherIdentificationen_US
dc.subject.otherCapricolumen_US
dc.subject.otherGoatsen_US
dc.subject.otherPCRen_US
dc.titlePolymerase chain reaction and restriction endonuclease digestion for selected members of the ''Mycoplasma mycoides cluster'' and Mycoplasma putrefaciensen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1177/104063879700900213en_US
dc.identifier.pmid9211239-
dc.identifier.scopus0031113359-
dc.identifier.isiA1997XM72200013-
dc.contributor.authorscopusid35473510500-
dc.contributor.authorscopusid6603602022-
dc.contributor.authorscopusid7102022016-
dc.contributor.authorscopusid7403483205-
dc.contributor.authorscopusid7003836928-
dc.description.lastpage190en_US
dc.identifier.issue2-
dc.description.firstpage186en_US
dc.relation.volume9en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid1202192-
dc.contributor.daisngid1247734-
dc.contributor.daisngid32156367-
dc.contributor.daisngid687476-
dc.contributor.daisngid712942-
dc.description.numberofpages5en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Rodriguez, JL-
dc.contributor.wosstandardWOS:Ermel, RW-
dc.contributor.wosstandardWOS:Kenny, TP-
dc.contributor.wosstandardWOS:Brooks, DL-
dc.contributor.wosstandardWOS:DaMassa, AJ-
dc.date.coverdateEnero 1997en_US
dc.identifier.ulpgcen_US
dc.description.jcr1,057
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.fullNameRodríguez Navarro, José Luis-
Colección:Artículos
miniatura
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