Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/76462
DC FieldValueLanguage
dc.contributor.authorCerutti, Francescoen_US
dc.contributor.authorGiorda, Federicaen_US
dc.contributor.authorGrattarola, Carlaen_US
dc.contributor.authorMignone, Walteren_US
dc.contributor.authorBeltramo, Chiaraen_US
dc.contributor.authorKeck, Nicolasen_US
dc.contributor.authorLorusso, Alessioen_US
dc.contributor.authorDi Francesco, Gabriellaen_US
dc.contributor.authorDi Renzo, Ludovicaen_US
dc.contributor.authorDi Guardo, Giovannien_US
dc.contributor.authorGoria, Mariellaen_US
dc.contributor.authorMasoero, Lorettaen_US
dc.contributor.authorAcutis, Pier Luigien_US
dc.contributor.authorCasalone, Cristinaen_US
dc.contributor.authorPeletto, Simoneen_US
dc.date.accessioned2020-12-09T13:31:40Z-
dc.date.available2020-12-09T13:31:40Z-
dc.date.issued2020en_US
dc.identifier.issn2045-2322en_US
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/76462-
dc.description.abstractDolphin morbillivirus (DMV) is considered an emerging threat having caused several epidemics worldwide. Only few DMV genomes are publicly available. Here, we report the use of target enrichment directly from cetacean tissues to obtain novel DMV genome sequences, with sequence comparison and phylodynamic analysis. RNA from 15 tissue samples of cetaceans stranded along the Italian and French coasts (2008–2017) was purified and processed using custom probes (by bait hybridization) for target enrichment and sequenced on Illumina MiSeq. Data were mapped against the reference genome, and the novel sequences were aligned to the available genome sequences. The alignment was then used for phylogenetic and phylogeographic analysis using MrBayes and BEAST. We herein report that target enrichment by specific capture may be a successful strategy for whole-genome sequencing of DMV directly from field samples. By this strategy, 14 complete and one partially complete genomes were obtained, with reads mapping to the virus up to 98% and coverage up to 7800X. The phylogenetic tree well discriminated the Mediterranean and the NE-Atlantic strains, circulating in the Mediterranean Sea and causing two different epidemics (2008–2015 and 2014–2017, respectively), with a limited time overlap of the two strains, sharing a common ancestor approximately in 1998.en_US
dc.languageengen_US
dc.relation.ispartofScientific Reportsen_US
dc.sourceScientific Reports [EISSN 2045-2322], v. 10 (1), 20831, (Diciembre 2020)en_US
dc.subject240119 Zoología marinaen_US
dc.subject310907 Patologíaen_US
dc.subject.otherMetagenomicsen_US
dc.subject.otherPhylogeneticsen_US
dc.subject.otherViral epidemiologyen_US
dc.subject.otherViral evolutionen_US
dc.subject.otherDolphin morbillivirusen_US
dc.titleSpecific capture and whole-genome phylogeography of Dolphin morbillivirusen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1038/s41598-020-77835-zen_US
dc.identifier.scopus85096922658-
dc.contributor.authorscopusid55708887100-
dc.contributor.authorscopusid55972238400-
dc.contributor.authorscopusid6506971779-
dc.contributor.authorscopusid6506565983-
dc.contributor.authorscopusid26421895900-
dc.contributor.authorscopusid26321697600-
dc.contributor.authorscopusid57218216473-
dc.contributor.authorscopusid21533902400-
dc.contributor.authorscopusid57192422743-
dc.contributor.authorscopusid36860870100-
dc.contributor.authorscopusid55396432500-
dc.contributor.authorscopusid54786809100-
dc.contributor.authorscopusid23984168300-
dc.contributor.authorscopusid16177404700-
dc.contributor.authorscopusid22986339200-
dc.identifier.eissn2045-2322-
dc.identifier.issue1-
dc.relation.volume10en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.date.coverdateDiciembre 2020en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-VETen_US
dc.description.sjr1,24
dc.description.jcr4,379
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.fullNameGiorda, Federica-
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