Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/76219
Campo DC Valoridioma
dc.contributor.authorSieuwerts, A. M. Y.en_US
dc.contributor.authorMostert, B.en_US
dc.contributor.authorBolt-de Vries, J.en_US
dc.contributor.authorKraan, J.en_US
dc.contributor.authorDirix, L. Y.en_US
dc.contributor.authorvan Dam, P. A.en_US
dc.contributor.authorvan Galen, A.en_US
dc.contributor.authorvan der Spoel, P.en_US
dc.contributor.authorRamírez-Moreno, Raquelen_US
dc.contributor.authorYu, J. X.en_US
dc.contributor.authorWang, Y.en_US
dc.contributor.authorGratama, J. W.en_US
dc.contributor.authorSleijfer, S.en_US
dc.contributor.authorFoekens, J. A.en_US
dc.contributor.authorMartens, J. W. M.en_US
dc.date.accessioned2020-12-02T11:19:33Z-
dc.date.available2020-12-02T11:19:33Z-
dc.date.issued2010en_US
dc.identifier.issn0008-5472en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/76219-
dc.description.abstractIntroduction: The enumeration of CTCs has already shown to bear clinical relevance as a prognostic and predictive factor in metastatic breast cancer. In addition to enumeration, isolation of CTCs enables their molecular characterization and thus holds great promise to establish association of their genetic profile with patient outcome and to identify potential drugable targets. In this study we established epithelial-specific mRNA and microRNA profiles in CTCs of patients with metastatic breast cancer, compared these profiles to the profiles measured in corresponding primary tumors, and determined their association with clinical parameters. Study design: For this study we included 50 breast cancer patients, of which 32 presented themselves with over 5 CTCs at the time of metastatic disease. From 14 of these patients with more than 5 CTCs at the time of metastatic disease also the primary tumor at time of breast cancer diagnosis was evaluated. Total RNA was extracted 1) from blood of the 50 patients with metastatic disease after EpCAM-based enrichment of 7.5 mL whole blood with the CellSearch™ Profile Kit [Veridex LCC], 2) from 14 unprocessed whole blood preparations from healthy blood donors, and 3) from 14 primary tumors. Gene transcript levels of CTC-specific and potentially clinically relevant mRNAs and microRNAs were compared in CTCs isolated at time of metastatic disease and the corresponding primary tumors. In addition, the association of these transcript levels with clinical data was assessed. Results: We identified 24 mRNA and 14 microRNAs more abundantly expressed in CellSearch-enriched fractions from patients with at least 5 CTCs compared with those without CTCs and/or compared with unprocessed whole blood prior to CellSearch enrichment (Mann-Whitney U-test P<0.05). In addition, when comparing transcript levels present in CTCs during metastatic disease and those measured in the corresponding primary tumor, potentially clinically relevant discrepancies were observed. Findings of interest included changes in transcript levels of genes such as ESR1, ERBB2, TOP2A and MGB1, and in genes associated with proliferation and EMT. Finally, associations were observed between transcript levels measured in CTC preparations and clinical data like nodal status and size of the primary tumor. Conclusion: Our results show that molecular characterization of CTCs is feasible and has potential for a more tailored clinical approach above CTC enumeration in the treatment of metastatic breast cancer patients.en_US
dc.languageengen_US
dc.relation.ispartofCancer research (Chicago, Ill.)en_US
dc.sourceCancer Research [ISSN 0008-5472], v. 70 (sup. 24), Abstract P3-02-05, (Diciembre 2010)en_US
dc.subject320101 Oncologíaen_US
dc.subject32 Ciencias médicasen_US
dc.subject.otherTumorsen_US
dc.subject.otherMetastatic Diseaseen_US
dc.titleEvaluation of Gene Transcripts in Primary Tumors at Time of Diagnosis and Circulating Tumor Cells (CTCs) at Time of Metastatic Diseaseen_US
dc.typeinfo:eu-repo/semantics/conferenceObjecten_US
dc.typeConference posteren_US
dc.relation.conference33th Annual CTRC‐AACR San Antonio Breast Cancer Symposiumen_US
dc.identifier.doi10.1158/0008-5472.SABCS10-P3-02-05en_US
dc.identifier.isi000209695801177-
dc.identifier.eissn1538-7445-
dc.relation.volume70en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Póster de congresosen_US
dc.contributor.daisngid24294284-
dc.contributor.daisngid1279918-
dc.contributor.daisngid1872513-
dc.contributor.daisngid266668-
dc.contributor.daisngid17934-
dc.contributor.daisngid2279472-
dc.contributor.daisngid2068016-
dc.contributor.daisngid3158692-
dc.contributor.daisngid2567033-
dc.contributor.daisngid13750744-
dc.contributor.daisngid13881392-
dc.contributor.daisngid32888-
dc.contributor.daisngid43725-
dc.contributor.daisngid20416-
dc.contributor.daisngid34295-
dc.description.numberofpages1en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Sieuwerts, AM-
dc.contributor.wosstandardWOS:Mostert, B-
dc.contributor.wosstandardWOS:Bolt-de Vries, J-
dc.contributor.wosstandardWOS:Kraan, J-
dc.contributor.wosstandardWOS:Dirix, LY-
dc.contributor.wosstandardWOS:van Dam, PA-
dc.contributor.wosstandardWOS:van Galen, A-
dc.contributor.wosstandardWOS:van der Spoel, P-
dc.contributor.wosstandardWOS:Ramirez-Moreno, R-
dc.contributor.wosstandardWOS:Yu, JX-
dc.contributor.wosstandardWOS:Wang, Y-
dc.contributor.wosstandardWOS:Gratama, JW-
dc.contributor.wosstandardWOS:Sleijfer, S-
dc.contributor.wosstandardWOS:Foekens, JA-
dc.contributor.wosstandardWOS:Martens, JWM-
dc.date.coverdateDiciembre 2010en_US
dc.identifier.supplement24-
dc.identifier.abstractidP3-02-05-
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-MEDen_US
dc.description.jcr8,234
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.event.eventsstartdate08-12-2010-
crisitem.event.eventsenddate12-12-2010-
crisitem.author.deptGIR IUIBS: Farmacología Molecular y Traslacional-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameRamirez Moreno,Raquel-
Colección:Póster de congreso
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