Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/76196
Title: Blockade Of Prostaglandins And Nitric Oxide In-vivo Reduces State 3 Mitochondrial Respiration In Human Skeletal Muscle
Authors: Fuentes-Nieto, Teresa
Hellsten, Ylva
Saltin, Bengt
Boushel ---,Robert 
UNESCO Clasification: 32 Ciencias médicas
241117 Fisiología de la respiración
Keywords: Prostaglandin
Nitric oxide
Mithocondrial respiration
Issue Date: 2010
Journal: Medicine and Science in Sports and Exercise 
Conference: 57th Annual Meeting of the American-College-Sports-Medicine/Inaugural World Congress on Exercise is Medicine 
Abstract: PURPOSE: The purpose of this study was to examine in humans the independent and combined effect of NO and PG blockade with L-NMMA and Indomethacin (Indo) on mitochondrial respiration in muscle from biopsies of the vastus lateralis (VL) following knee extension (KE) exercise. METHODS: Mitochondrial respiration was measured ex-vivo by high resolution respirometry in saponin-permeabilized fibers following 6 min KE in control (CON, n=8), arterial infusion of LNMMA (n=4) and Indo (n=4) randomized in order, followed by combined inhibition of NO and PG (L-NMMA + Indo, n=8). RESULTS. ADP-stimulated state 3 respiration with substrates for complex I (glutamate, malate) was reduced 50% by Indo compared to CON. State 3 O2 flux with convergent electron input through both complex I and II with addition of succinate was equally reduced with Indo (20%) and L-NMMA + Indo (15%) compared to CON and LNMMA alone. Uncoupled O2 flux determined by titration of the ionophore FCCP was not increased above state 3 respiration with Indo suggesting tight coupling to oxidative phosphorylation. Graded titrations of L-NMMA into the respirometer in CON muscle reduced state 3 respiration by 12% only at a supra-physiological dose, while titration of Indo caused a linear reduction at all doses within and above the physiological range. CONCLUSION: The results indicate that inhibition of PG by indomethacin inhibits state 3 mitochondrial respiration primarily at complex I of the respiratory chain. This effect may in part explain the in-vivo reduction in muscle O2 uptake during exercise with double blockade of NO and PG, since blockade of NO by L-NMMA counteracts the inhibitory effect of Indo.
URI: http://hdl.handle.net/10553/76196
ISSN: 0195-9131
DOI: 10.1249/01.MSS.0000386393.75174.55
Source: Medicine And Science In Sports And Exercise [ISSN 0195-9131], v. 42 (5), (sup. 1), p. 147, (Mayo 2010)
Appears in Collections:Actas de congresos
Thumbnail
Adobe PDF (104,41 kB)
Show full item record

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.