Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/75518
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dc.contributor.authorRamos-Pinto, Lourençoen_US
dc.contributor.authorAzeredo, Ritaen_US
dc.contributor.authorSilva, Carlotaen_US
dc.contributor.authorConceição, Luís E. C.en_US
dc.contributor.authorDias, Jorgeen_US
dc.contributor.authorMontero Vítores, Danielen_US
dc.contributor.authorTorrecillas Burriel,Silviaen_US
dc.contributor.authorSilva, Tomé S.en_US
dc.contributor.authorCostas, Benjaminen_US
dc.date.accessioned2020-11-13T13:21:08Z-
dc.date.available2020-11-13T13:21:08Z-
dc.date.issued2020en_US
dc.identifier.issn1664-3224en_US
dc.identifier.urihttp://hdl.handle.net/10553/75518-
dc.description.abstractSeveral amino acids (AA) are known to regulate key metabolic pathways that are crucial for immune responses. In particular, arginine (ARG) appears to have important roles regarding immune modulation since it is required for macrophage responses and lymphocyte development. Moreover, citrulline (CIT) is a precursor of arginine, and it was reported as an alternative to ARG for improving macrophage function in mammals. The present study aimed to explore the effects of dietary ARG and CIT supplementation on the gilthead seabream (Sparus aurata) immune status. Triplicate groups of fish (23.1 ± 0.4 g) were either fed a control diet (CTRL) with a balanced AA profile, or the CTRL diet supplemented with graded levels of ARG or CIT (i.e., 0.5 and 1% of feed; ARG1, CIT1, ARG2, and CIT2, respectively). After 2 and 4 weeks of feeding, fish were euthanized and blood was collected for blood smears, plasma for humoral immune parameters and shotgun proteomics, and head-kidney tissue for the measurement of health-related transcripts. A total of 94 proteins were identified in the plasma of all treatments. Among them, components of the complement system, apolipoproteins, as well as some glycoproteins were found to be highly abundant. After performing a PLS of the expressed proteins, differences between the two sampling points were observed. In this regard, component 1 (61%) was correlated with the effect of sampling time, whereas component 2 (18%) seemed associated to individual variability within diet. Gilthead seabream fed ARG2 and CIT2 at 4 weeks were more distant than fish fed all dietary treatments at 2 weeks and fish fed the CTRL diet at 4 weeks. Therefore, data suggest that the modulatory effects of AA supplementation at the proteome level were more effective after 4 weeks of feeding and at the higher inclusion level (i.e., 1% of feed). The bactericidal activity increased in fish fed the highest supplementation level of both AAs after 4 weeks. Peripheral monocyte numbers correlated positively with nitric oxide, which showed an increasing trend in a dose-dependent manner. The colony-stimulating factor 1 receptor tended to be up-regulated at the final sampling point regardless of dietary treatments. Data from this study point to an immunostimulatory effect of dietary ARG or CIT supplementation after 4 weeks of feeding in the gilthead seabream, particularly when supplemented at a 1% inclusion level.en_US
dc.languageengen_US
dc.relationALISSA (ALG-01-0247-FEDER-3520)en_US
dc.relationUID/Multi/04423/2019en_US
dc.relationIF/00197/2015en_US
dc.relationIF/00482/2014en_US
dc.relation.ispartofFrontiers in Immunologyen_US
dc.sourceFrontiers in Immunology [ISSN 1664-3224], v. 11, 1544 (agosto 2020)en_US
dc.subject251092 Acuicultura marinaen_US
dc.subject.otherAmino acidsen_US
dc.subject.otherImmunologyen_US
dc.subject.otherSustainable aquacultureen_US
dc.subject.otherFunctional feedsen_US
dc.subject.otherPlasma proteomeen_US
dc.titleShort-Term Supplementation of Dietary Arginine and Citrulline Modulates Gilthead Seabream (Sparus aurata) Immune Statusen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fimmu.2020.01544en_US
dc.relation.volume11en_US
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.description.numberofpages16en_US
dc.utils.revisionen_US
dc.date.coverdateAgosto 2020en_US
dc.identifier.ulpgcen_US
dc.contributor.buulpgcBU-BASen_US
dc.description.sjr2,646-
dc.description.jcr7,561-
dc.description.sjrqQ1-
dc.description.jcrqQ1-
dc.description.scieSCIE-
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.deptDepartamento de Biología-
crisitem.author.deptGIR Grupo de Investigación en Acuicultura-
crisitem.author.deptIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.orcid0000-0002-4358-2157-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.parentorgIU de Investigación en Acuicultura Sostenible y Ec-
crisitem.author.fullNameMontero Vítores, Daniel-
crisitem.author.fullNameTorrecillas Burriel, Silvia-
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