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dc.contributor.authorMorchón, R.en_US
dc.contributor.authorBargues, M. D.en_US
dc.contributor.authorLatorre-Estivalis, J. M.en_US
dc.contributor.authorPou-Barreto, C.en_US
dc.contributor.authorMelero-Alcibar, R.en_US
dc.contributor.authorMoreno, M.en_US
dc.contributor.authorValladares, B.en_US
dc.contributor.authorMolina, R.en_US
dc.contributor.authorMontoya Alonso, José Albertoen_US
dc.contributor.authorMas-Coma, S.en_US
dc.contributor.authorSimón, F.en_US
dc.description.abstractDirofilaria immitis is the causal agent of heartworm diseases and of human pulmonary dirofilariosis. The infection is transmitted by several species of culicid mosquitoes that are frequently able to bite both animal reservoirs and humans. Canary Islands (Spain) constitute a well documented endemic area of canine dirofilariosis in which the mosquito species involved in the transmission of D. immitis are not known. The objectives of the present work were the identification of vectors of this parasite in Canary Islands and their molecular characterization. A total of 1219 female mosquitoes were captured. The most abundant species was Culex theileri (52.26%) followed by Cx. pipiens (35.44%), Anopheles cinereus hispaniola (6.23%), Culiseta longiareolata (5.74%), and Culex laticintus (0.33%). PCR was applied for the detection of larval D. immitis DNA in mosquitoes. D. immitis DNA was observed in the abdomen of one Cx. theileri female: 0.082% of the entire mosquito population and 0.17% in Cx. theileri. A molecular identification of Cx. theileri, the potential mosquito vector of dirofilariosis in this zoonotic focus in Canary Islands of Spain, has been made for first time based on sequences of the 18S rRNA gene, the second internal transcribed spacer (ITS2) of ribosomal DNA and the barcode region of the cytochrome c oxidase I (cox1) gene of mitochondrial DNA, allowing a broad mosquito molecular basis for future populations genetic analyses of this vector species. Parasitological and entomological molecular results suggest that Cx. theileri is a potential natural vector of D. immitis in Canary Islands.en_US
dc.relation.ispartofJournal of Clinical & Experimental Pathologyen_US
dc.sourceJournal of Clinical & Experimental Pathology [ISSN 2161-0681], S3: 001 (2011)en_US
dc.subject310907 Patologíaen_US
dc.subject.otherDirofilaria immitisen_US
dc.subject.otherCulex theilerien_US
dc.subject.otherCanary Islandsen_US
dc.subject.other18S rRNA geneen_US
dc.subject.otherITS-2 rDNAen_US
dc.subject.otherBarcode region mtDNAen_US
dc.titleMolecular characterization of Culex Theileri from Canary Islands, Spain, a potential vector of Dirofilaria Immitisen_US
dc.investigacionCiencias de la Saluden_US
item.fulltextCon texto completo-
item.grantfulltextopen- Veterinaria e Investigación Terapeútica- de Investigaciones Biomédicas y Sanitarias- de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos- de Investigaciones Biomédicas y Sanitarias- Alonso, José Alberto-
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