Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/74508
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dc.contributor.authorCarvalho, Isabelen_US
dc.contributor.authorTejedor Junco, María Teresaen_US
dc.contributor.authorGonzález Martín, Margarita Rosaen_US
dc.contributor.authorCorbera Sánchez, Juan Albertoen_US
dc.contributor.authorSilva, Vanessaen_US
dc.contributor.authorIgrejas, Gilbertoen_US
dc.contributor.authorTorres, Carmenen_US
dc.contributor.authorPoeta, Patríciaen_US
dc.date.accessioned2020-09-21T11:18:03Z-
dc.date.available2020-09-21T11:18:03Z-
dc.date.issued2020en_US
dc.identifier.issn2076-2615en_US
dc.identifier.urihttp://hdl.handle.net/10553/74508-
dc.description.abstractThis work aimed to determine the carriage rate of ESBL-producing Escherichia coli as well as their genetic characteristics in camels from the Canary Islands, Spain. Methods: Fecal samples were recovered from 58 healthy camels from Gran Canaria (n = 32) and Fuerteventura Islands (n = 26) during July 2019. They were seeded on MacConkey (MC) agar no supplemented and supplemented (MC + CTX) with cefotaxime (2 µg/mL). Antimicrobial susceptibility was determined by disk diffusion test (CLSI, 2018). The presence of blaCTX-M, blaSHV, blaTEM,blaCMY-2 and blaOXA-1/48 genes was tested by PCR/sequencing. Furthermore, the mcr-1 (colistin resistance), tetA/tetB (tetracycline resistance), int1 (integrase of class 1 integrons) and stx1,2 genes were analyzed. Phylogenetic groups and sequence types were determined by specific-PCR/sequencing for selected isolates. Results: E. coli was obtained from all the 58 camels in MC media (100%) and in five of them in MC + CTX media (8.6%). Furthermore, 63.8% of E. coli isolates recovered from MC agar were susceptible to all the antibiotics tested. The five E. coli isolates recovered from MC + CTX media were characterized and two of them were ESBL-producers (3.4%). Both ESBL-producer isolates carried the blaCTX-M-15 gene and belonged to the lineages ST3018 (phylogroup A) and ST69 (phylogroup B1). The 3 ESBL-negative isolates recovered from MC-CTX plates were ascribed to phylogroup-B1. Conclusions: Camels can be a source of ESBL-producer bacteria, containing the widespread blaCTX-M-15 gene associated with the lineages ST3018 and ST69.en_US
dc.languagespaen_US
dc.relation.ispartofAnimalsen_US
dc.sourceAnimals [2076-2615], vol. 10(8)en_US
dc.subject310905 Microbiologíaen_US
dc.subject310907 Patologíaen_US
dc.subject.otherAntimicrobial resistanceen_US
dc.subject.otherExtended-spectrum β-lactamasesen_US
dc.subject.otherEscherichia colien_US
dc.subject.otherCamelsen_US
dc.titleEscherichia coli Producing Extended-Spectrum β-lactamases (ESBL) from Domestic Camels in the Canary Islands: A One Health Approachen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.3390/ani10081295en_US
dc.identifier.issue8-
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgces
dc.description.sjr0,584
dc.description.jcr2,752
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Ciencias Clínicas-
crisitem.author.deptGIR IUIBS: Trypanosomosis, Resistencia a Antibióticos y Medicina Animal-
crisitem.author.deptIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.orcid0000-0003-2387-1426-
crisitem.author.orcid0000-0002-4457-2321-
crisitem.author.orcid0000-0001-7812-2065-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.parentorgIU de Investigaciones Biomédicas y Sanitarias-
crisitem.author.fullNameTejedor Junco, María Teresa-
crisitem.author.fullNameGonzález Martín, Margarita Rosa-
crisitem.author.fullNameCorbera Sánchez, Juan Alberto-
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