Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/73991
DC FieldValueLanguage
dc.contributor.authorSuvorova, Elena S.en_US
dc.contributor.authorCroken, Matthewen_US
dc.contributor.authorKratzer, Stellaen_US
dc.contributor.authorTing, Li-Minen_US
dc.contributor.authorConde De Felipe, Magnolia Maríaen_US
dc.contributor.authorBalu, Bharathen_US
dc.contributor.authorMarkillie, Meng L.en_US
dc.contributor.authorWeiss, Louis M.en_US
dc.contributor.authorKim, Kamien_US
dc.contributor.authorWhite, Michael W.en_US
dc.date.accessioned2020-08-06T08:54:04Z-
dc.date.available2020-08-06T08:54:04Z-
dc.date.issued2013en_US
dc.identifier.issn1553-7390en_US
dc.identifier.otherWoS-
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/73991-
dc.description.abstractIn the G1 phase of the cell division cycle, eukaryotic cells prepare many of the resources necessary for a new round of growth including renewal of the transcriptional and protein synthetic capacities and building the machinery for chromosome replication. The function of G1 has an early evolutionary origin and is preserved in single and multicellular organisms, although the regulatory mechanisms conducting G1 specific functions are only understood in a few model eukaryotes. Here we describe a new G1 mutant from an ancient family of apicomplexan protozoans. Toxoplasma gondii temperature-sensitive mutant 12-109C6 conditionally arrests in the G1 phase due to a single point mutation in a novel protein containing a single RNA-recognition-motif (TgRRM1). The resulting tyrosine to asparagine amino acid change in TgRRM1 causes severe temperature instability that generates an effective null phenotype for this protein when the mutant is shifted to the restrictive temperature. Orthologs of TgRRM1 are widely conserved in diverse eukaryote lineages, and the human counterpart (RBM42) can functionally replace the missing Toxoplasma factor. Transcriptome studies demonstrate that gene expression is downregulated in the mutant at the restrictive temperature due to a severe defect in splicing that affects both cell cycle and constitutively expressed mRNAs. The interaction of TgRRM1 with factors of the tri-SNP complex (U4/U6 & U5 snRNPs) indicate this factor may be required to assemble an active spliceosome. Thus, the TgRRM1 family of proteins is an unrecognized and evolutionarily conserved class of splicing regulators. This study demonstrates investigations into diverse unicellular eukaryotes, like the Apicomplexa, have the potential to yield new insights into important mechanisms conserved across modern eukaryotic kingdoms.en_US
dc.languageengen_US
dc.relation.ispartofPLoS Geneticsen_US
dc.sourcePlos Genetics [ISSN 1553-7404], v. 9 (2), e1003305, (Febrero 2013)en_US
dc.subject310907 Patologíaen_US
dc.subject320712 Parasitologíaen_US
dc.subject.otherParasite Toxoplasma-Gondiien_US
dc.subject.otherGene-Expressionen_US
dc.subject.otherFission Yeasten_US
dc.subject.otherSaccharomyces-Cerevisiaeen_US
dc.subject.otherDivision Cycleen_US
dc.subject.otherPlasmodiumen_US
dc.subject.otherProteinen_US
dc.subject.otherIdentificationen_US
dc.subject.otherSpliceosomeen_US
dc.subject.otherProteomicsen_US
dc.titleDiscovery of a Splicing Regulator Required for Cell Cycle Progressionen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1371/journal.pgen.1003305en_US
dc.identifier.scopus84874779572-
dc.identifier.isi000315638300055-
dc.contributor.authorscopusid7004851161-
dc.contributor.authorscopusid43860899900-
dc.contributor.authorscopusid54415893800-
dc.contributor.authorscopusid35839739600-
dc.contributor.authorscopusid55617921100-
dc.contributor.authorscopusid6507673904-
dc.contributor.authorscopusid6507287138-
dc.contributor.authorscopusid7403207276-
dc.contributor.authorscopusid7409320892-
dc.contributor.authorscopusid35723203600-
dc.identifier.eissn1553-7404-
dc.identifier.issue2-
dc.relation.volume9en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid1440703-
dc.contributor.daisngid3485905-
dc.contributor.daisngid4417588-
dc.contributor.daisngid1210666-
dc.contributor.daisngid5732759-
dc.contributor.daisngid1538840-
dc.contributor.daisngid7078665-
dc.contributor.daisngid72430-
dc.contributor.daisngid455477-
dc.contributor.daisngid4653189-
dc.description.numberofpages18en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Suvorova, ES-
dc.contributor.wosstandardWOS:Croken, M-
dc.contributor.wosstandardWOS:Kratzer, S-
dc.contributor.wosstandardWOS:Ting, LM-
dc.contributor.wosstandardWOS:de Felipe, MC-
dc.contributor.wosstandardWOS:Balu, B-
dc.contributor.wosstandardWOS:Markillie, ML-
dc.contributor.wosstandardWOS:Weiss, LM-
dc.contributor.wosstandardWOS:Kim, K-
dc.contributor.wosstandardWOS:White, MW-
dc.date.coverdateFebrero 2013en_US
dc.identifier.ulpgces
dc.description.sjr6,605
dc.description.jcr8,167
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.fulltextCon texto completo-
item.grantfulltextopen-
crisitem.author.deptGIR Parasitología, dermatologia y biopatologia veterinaria-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, Bromatología y Tecnología de Los Alimentos-
crisitem.author.fullNameConde De Felipe,Magnolia María-
Appears in Collections:Artículos
Thumbnail
pdf
Adobe PDF (511,06 kB)
Show simple item record

SCOPUSTM   
Citations

32
checked on Oct 13, 2024

WEB OF SCIENCETM
Citations

32
checked on Oct 13, 2024

Page view(s)

111
checked on Sep 21, 2024

Download(s)

95
checked on Sep 21, 2024

Google ScholarTM

Check

Altmetric


Share



Export metadata



Items in accedaCRIS are protected by copyright, with all rights reserved, unless otherwise indicated.