Identificador persistente para citar o vincular este elemento: http://hdl.handle.net/10553/73929
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dc.contributor.authorCastillo, Yaiza M.en_US
dc.contributor.authorSebastián Caumel, Martaen_US
dc.contributor.authorForn, Ireneen_US
dc.contributor.authorGrimsley, Nigelen_US
dc.contributor.authorYau, Shereeen_US
dc.contributor.authorMoraru, Cristinaen_US
dc.contributor.authorVaqué, Dolorsen_US
dc.date.accessioned2020-08-03T09:08:23Z-
dc.date.available2020-08-03T09:08:23Z-
dc.date.issued2020en_US
dc.identifier.issn1664-302Xen_US
dc.identifier.otherScopus-
dc.identifier.urihttp://hdl.handle.net/10553/73929-
dc.description.abstractOne of the major challenges in viral ecology is to assess the impact of viruses in controlling the abundance of specific hosts in the environment. To this end, techniques that enable the detection and quantification of virus-host interactions at the single-cell level are essential. With this goal in mind, we implemented virus fluorescence in situ hybridization (VirusFISH) using as a model the marine picoeukaryote Ostreococcus tauri and its virus Ostreococcus tauri virus 5 (OtV5). VirusFISH allowed the visualization and quantification of the proportion of infected cells during an infection cycle in experimental conditions. We were also able to quantify the abundance of free viruses released during cell lysis, discriminating OtV5 from other mid-level fluorescence phages in our non-axenic infected culture that were not easily distinguishable with flow cytometry. Our results showed that although the major lysis of the culture occurred between 24 and 48 h after OtV5 inoculation, some new viruses were already produced between 8 and 24 h. With this work, we demonstrate that VirusFISH is a promising technique to study specific virus-host interactions in non-axenic cultures and establish a framework for its application in complex natural communities.en_US
dc.languageengen_US
dc.relation.ispartofFrontiers in Microbiologyen_US
dc.sourceFrontiers in Microbiology [EISSN 1664-302X], v. 11, (Julio 2020)en_US
dc.subject2414 Microbiologíaen_US
dc.subject.otherCulture System
dc.subject.otherMarine Picoeukaryote
dc.subject.otherOstreococcus Tauri
dc.subject.otherOstreococcus Tauri Virus 5
dc.subject.otherVirus Fluorescence In Situ Hybridization
dc.subject.otherVirus-Host Interactions
dc.titleVisualization of Viral Infection Dynamics in a Unicellular Eukaryote and Quantification of Viral Production Using Virus Fluorescence in situ Hybridizationen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.3389/fmicb.2020.01559en_US
dc.identifier.scopus85088514757-
dc.contributor.authorscopusid57193991947-
dc.contributor.authorscopusid14031974200-
dc.contributor.authorscopusid14055741200-
dc.contributor.authorscopusid56658523200-
dc.contributor.authorscopusid36648249700-
dc.contributor.authorscopusid36476532000-
dc.contributor.authorscopusid7003841654-
dc.identifier.eissn1664-302X-
dc.relation.volume11en_US
dc.type2Artículoen_US
dc.description.numberofpages11en_US
dc.utils.revision
dc.date.coverdateJulio 2020en_US
dc.identifier.ulpgces
dc.description.sjr1,701
dc.description.jcr5,64
dc.description.sjrqQ1
dc.description.jcrqQ1
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.fullNameSebastián Caumel, Marta-
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