Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/73893
DC FieldValueLanguage
dc.contributor.authorMalama, Elenien_US
dc.contributor.authorHoffmann-Koehler, Peggyen_US
dc.contributor.authorBiedermann, Insaen_US
dc.contributor.authorKoopmann, Regineen_US
dc.contributor.authorKruecken, Juergenen_US
dc.contributor.authorMolina Caballero, José Manuelen_US
dc.contributor.authorMartinez Moreno, Alvaroen_US
dc.contributor.authorvon Samson-Himmelstjerna, Georgen_US
dc.contributor.authorSotiraki, Smaragdaen_US
dc.contributor.authorDemeler, Janinaen_US
dc.date.accessioned2020-07-31T10:35:19Z-
dc.date.available2020-07-31T10:35:19Z-
dc.date.issued2014en_US
dc.identifier.issn0932-0113en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/73893-
dc.description.abstractTeladorsagia circumcincta is among the most important gastrointestinal parasites in small ruminants and the predominant species in Southern European goats. Parasite control is largely based on metaphylactic/preventative treatments, which is often seen as non-sustainable anymore. The reasons are increased consumer demand to reduce chemicals in livestock production and anthelmintic resistance against the common drugs. This study aimed at the development of a T. circumcincta-enzyme-linked immunosorbent assay (ELISA) specifically for goats. Samples were obtained from goats raised parasite-free or infected experimentally. Sampling continued during the following pasture season and housing period. The sensitivity for the use in bulk milk samples as an indicator of T. circumcincta infection levels in grazing goats was examined. The ELISA enables clear differentiation of negative and positive animals. With a specificity of 100 % negative cut-off values for serum and milk were 0.294 and 0.228 (sensitivity, 95 %). Positive cut-off values (sensitivity, 90 %) were 0.606 (serum) and 0.419 (milk), while a sensitivity of 95 % resulted in 0.509 and 0.363, respectively. The grey-zone between negative/positive cut-offs was introduced to deal with animals in pre-patency and decreasing antibody levels after infection. There was no cross reactivity for Trichostrongylus colubriformis and Cooperia oncophora while for Haemonchus contortus and Fasciola hepatica it cannot be fully excluded currently. In bulk milk samples, 5 % of the milk had to be contributed from animals infected with T. circumcincta to be detected as positive. The results derived from experimentally and naturally infected as well as parasite na < ve animals indicate the potential of the ELISA to be used in targeted anthelmintic treatment regimes in goats.en_US
dc.languageengen_US
dc.relationWorkshop/Conference Id Ecost-Meeting-Fa0805-190312-016348en_US
dc.relation.ispartofParasitology Researchen_US
dc.sourceParasitology Research [ISSN 0932-0113], v. 113 (10), p. 3651-3660, (Octubre 2014)en_US
dc.subject310907 Patologíaen_US
dc.subject3104 Producción Animalen_US
dc.subject.otherTeladorsagiaen_US
dc.subject.otherELISAen_US
dc.subject.otherGoaten_US
dc.subject.otherGastrointestinal Nematodesen_US
dc.subject.otherDiagnosticen_US
dc.subject.otherMilken_US
dc.subject.otherSerumen_US
dc.titleDevelopment of a milk and serum ELISA test for the detection of Teladorsagia circumcincta antibodies in goats using experimentally and naturally infected animalsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1007/s00436-014-4030-6en_US
dc.identifier.scopus84918806902-
dc.identifier.isi000342453800013-
dc.contributor.authorscopusid55479366800-
dc.contributor.authorscopusid21739035800-
dc.contributor.authorscopusid56268902500-
dc.contributor.authorscopusid55976976200-
dc.contributor.authorscopusid6603005189-
dc.contributor.authorscopusid55731962600-
dc.contributor.authorscopusid56268809000-
dc.contributor.authorscopusid55667396600-
dc.contributor.authorscopusid6602529473-
dc.contributor.authorscopusid15833967300-
dc.identifier.eissn1432-1955-
dc.description.lastpage3660en_US
dc.identifier.issue10-
dc.description.firstpage3651en_US
dc.relation.volume113en_US
dc.investigacionCiencias de la Saluden_US
dc.type2Artículoen_US
dc.contributor.daisngid1680954-
dc.contributor.daisngid7791470-
dc.contributor.daisngid7205182-
dc.contributor.daisngid1653871-
dc.contributor.daisngid498321-
dc.contributor.daisngid742292-
dc.contributor.daisngid984836-
dc.contributor.daisngid144314-
dc.contributor.daisngid725784-
dc.contributor.daisngid664554-
dc.description.numberofpages10en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Malama, E-
dc.contributor.wosstandardWOS:Hoffmann-Kohler, P-
dc.contributor.wosstandardWOS:Biedermann, I-
dc.contributor.wosstandardWOS:Koopmann, R-
dc.contributor.wosstandardWOS:Krucken, J-
dc.contributor.wosstandardWOS:Molina, JM-
dc.contributor.wosstandardWOS:Moreno, AM-
dc.contributor.wosstandardWOS:von Samson-Himmelstjerna, G-
dc.contributor.wosstandardWOS:Sotiraki, S-
dc.contributor.wosstandardWOS:Demeler, J-
dc.date.coverdateEnero 2014en_US
item.fulltextSin texto completo-
item.grantfulltextnone-
crisitem.author.deptDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.deptParasitología, dermatologia y biopatologia veterinaria-
crisitem.author.parentorgDepartamento de Patología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
crisitem.author.fullNameMolina Caballero, José Manuel-
crisitem.author.departamentoPatología Animal, Producción Animal, y Ciencia y Tecnología deLos Alimentos-
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