Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/73608
DC FieldValueLanguage
dc.contributor.authorHamdan, Mariamen_US
dc.contributor.authorMoyano, Francisco J.en_US
dc.contributor.authorSchuhardt, Dominiqueen_US
dc.date.accessioned2020-07-02T12:15:06Z-
dc.date.available2020-07-02T12:15:06Z-
dc.date.issued2009en_US
dc.identifier.issn0022-5142en_US
dc.identifier.otherWoS-
dc.identifier.urihttp://hdl.handle.net/10553/73608-
dc.description.abstractBACKGROUND: Although several types of in vitro digestibility assays have been applied to nutritional evaluation of feeds for aquatic organisms, all of them are based on the use of closed reactors and do not simulate the gastric phase of the digestion. Our objective was to evaluate the suitability of a gastrointestinal model based on the use of a digestion cell provided by a semi-permeable reaction chamber, which allows continuous removal of digestion products as they are produced. We tested the effects of some factors, like the inclusion of a gastric phase, reaction temperature or bile salts on the hydrolysis of feed proteins by fish enzymes.RESULTS: We found that the most suitable operational conditions to simulate the digestion process must include a short acid pre-digestion as well as the use of bile salts in the reaction mixture. Acid pre-digestion resulted in a significant increase in the liberation of amino acids which represented more than twice that measured when using a single phase. The addition of two bile salts (45 mu mol L-1 sodium taurocholate + chenodesoxycolate) resulted in almost a threefold increase in the hydrolysis of feed protein. The use of the described open system also allows the evaluation of carbohydrate hydrolysis as well as determination of residual undigested matter, in a similar manner to that carried out in ruminants with the DAISY system.CONCLUSION: Results suggest the system can be a very suitable model for evaluation of bioaccessibility in fish feeds. (C) 2009 Society of Chemical Industryen_US
dc.languageengen_US
dc.relation.ispartofJournal of the Science of Food and Agricultureen_US
dc.sourceJournal Of The Science Of Food And Agriculture [ISSN 0022-5142], v. 89 (7), p. 1195-1201, (Mayo 2009)en_US
dc.subject251092 Acuicultura marinaen_US
dc.subject.otherVitro Protein Digestionen_US
dc.subject.otherIn-Vitroen_US
dc.subject.otherSmall-Intestineen_US
dc.subject.otherPyloric Cecaen_US
dc.subject.otherDigestibilityen_US
dc.subject.otherPurificationen_US
dc.subject.otherProteasesen_US
dc.subject.otherTrypsinen_US
dc.subject.otherStarchen_US
dc.subject.otherAciden_US
dc.subject.otherGastrointestinal Modelen_US
dc.subject.otherIn Vitro Digestionen_US
dc.subject.otherFishen_US
dc.subject.otherProteinen_US
dc.titleOptimization of a gastrointestinal model applicable to the evaluation of bioaccessibility in fish feedsen_US
dc.typeinfo:eu-repo/semantics/Articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1002/jsfa.3574en_US
dc.identifier.scopus65649126327-
dc.identifier.isi000265584900014-
dc.contributor.authorscopusid26537327100-
dc.contributor.authorscopusid6603769397-
dc.contributor.authorscopusid26538020600-
dc.identifier.eissn1097-0010-
dc.description.lastpage1201en_US
dc.identifier.issue7-
dc.description.firstpage1195en_US
dc.relation.volume89en_US
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.contributor.daisngid7437947-
dc.contributor.daisngid425906-
dc.contributor.daisngid16999016-
dc.description.numberofpages7en_US
dc.utils.revisionen_US
dc.contributor.wosstandardWOS:Hamdan, M-
dc.contributor.wosstandardWOS:Moyano, FJ-
dc.contributor.wosstandardWOS:Schuhardt, D-
dc.date.coverdateEnero 2009en_US
item.fulltextSin texto completo-
item.grantfulltextnone-
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