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Title: GDH activity and ammonium excretion in the marine mysid "Leptomysis lingvura": effects of age and starvation
Authors: Fernández-Urruzola, Igor 
Director: Packard, Theodore T. 
Gómez, May 
UNESCO Clasification: 251001 Oceanografía biológica
Keywords: Glutamate dehydrogenase
Ammonium excretion
Leptomysis lingvura
Body size
Issue Date: 2010
Project: Estudio de Un Nuevo Modelo Mecanistico Para El Metabolismo Del Zooplancton 
Abstract: Ammonium (NH4+) release by bacterial remineralization and heterotrophic grazers determines the regenerated fraction of phytoplankton productivity, so the measurement of NH4+ excretion in marine organisms is necessary to characterize both the magnitude and the efficiency of the nitrogen cycle. Glutamate dehydrogenase (GDH) is largely responsible for NH4+ formation in crustaceans and consequently should be useful in estimating NH4+ excretion by marine zooplankton.<br />Here, we address body size and starvation as sources of variability on the GDH to NH4+ excretion ratio (GDH/RNH4+). We found a strong correlation between the RNH4+ and the GDH activity (r2 = 0.87, n = 41) during growth. Since GDH activity maintained a linear relation (b = 0.93) and RNH4+ scaled exponentially (b =0.55) in well fed mysids, the GDH/RNH4+ ratio increased with size. However, the magnitude of its variation increased even more when adult mysids were starved. In this case, the GDH/RNH4+ ratio ranged from 11.23 to 102.41.
Description: Máster en Oceanografía
Faculty: Facultad de Ciencias del Mar
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