Please use this identifier to cite or link to this item: http://hdl.handle.net/10553/70714
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dc.contributor.authorGümüş Yılmaz, Gülçinen_US
dc.contributor.authorGómez Pinchetti, Juan Luisen_US
dc.contributor.authorCifuentes, Alejandroen_US
dc.contributor.authorHerrero, Miguelen_US
dc.contributor.authorIbáñez, Elenaen_US
dc.date.accessioned2020-03-06T12:23:18Z-
dc.date.available2020-03-06T12:23:18Z-
dc.date.issued2019en_US
dc.identifier.issn0003-2719en_US
dc.identifier.urihttp://hdl.handle.net/10553/70714-
dc.description.abstractSurfactant-mediated extraction (SME), pressurized liquid extraction (PLE), and enzyme-assisted extraction (EAE) have been compared to improve the isolation of phlorotannins from the brown algae Lobophora variegata. Enzymatic treatment with Alcalase 2.4 L FG, Carezyme 4500 L, protease from Streptomyces griseus, pectinase from Aspergillus niger, Celluclast 1.5 L, protease from Bacillus licheniformis; surfactant extraction with triacetin and guaiacol and PLE with ethanol:water as extracting solvent, have been studied in terms of total phenolic content by the Folin–Ciocalteu method and total phlorotannin content using the DMBA assay. The results showed that SME yields the highest amount of phenols and phlorotannins by using food grade guaiacol as the surfactant. An extraction protocol was developed to maximize the amount of extract obtained from L. variegata. The effects of various parameters such as the type of surfactant, efficacy of surfactant, and optimum pH, on the extraction efficiency of polyphenols were examined. The simultaneous use of the enzyme and surfactant was also investigated. However, a synergistic effect between the enzymes and the surfactant for the extraction of polyphenols has not been observed. Considering total phenols and total phlorotannins in the extract, the extraction yield were obtained for total phenols as SME > EAE > PLE and for total phlorotannins as SME > PLE > EAE.en_US
dc.languageengen_US
dc.relation.ispartofAnalytical lettersen_US
dc.sourceAnalytical Letters [0003-2719], vol. 52 (17), p. 2724-2740en_US
dc.subject339001 Biotecnología de microalgasen_US
dc.subject.otherBrown algeaen_US
dc.subject.otherLobophora variegataen_US
dc.subject.otherSurfactant-mediated extraction (SME)en_US
dc.titleComparison of Extraction Techniques and Surfactants for the Isolation of Total Polyphenols and Phlorotannins from the Brown Algae Lobophora variegataen_US
dc.typeinfo:eu-repo/semantics/articleen_US
dc.typeArticleen_US
dc.identifier.doi10.1080/00032719.2019.1597878en_US
dc.identifier.scopus2-s2.0-85063735794-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.contributor.orcid#NODATA#-
dc.description.lastpage2740-
dc.identifier.issue17-
dc.description.firstpage2724-
dc.relation.volume52-
dc.investigacionCienciasen_US
dc.type2Artículoen_US
dc.utils.revisionen_US
dc.identifier.ulpgces
dc.description.sjr0,3
dc.description.jcr1,467
dc.description.sjrqQ3
dc.description.jcrqQ4
dc.description.scieSCIE
item.grantfulltextnone-
item.fulltextSin texto completo-
crisitem.author.deptGIR IOCAG: Oceanografía Biológica y Algología Aplicada-
crisitem.author.deptIU de Oceanografía y Cambio Global-
crisitem.author.deptDepartamento de Biología-
crisitem.author.orcid0000-0003-4668-0462-
crisitem.author.parentorgIU de Oceanografía y Cambio Global-
crisitem.author.fullNameGómez Pinchetti, Juan Luis-
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